Sequences from confirmation amplicon


Confirmation sequences for line 400H09 and hit At5g20290 are shown.
Line ID 400H09
Locus At5g20290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6852291
Primers used for PCR LB13 - 8474, show primer details
   
Sequence Name 72-K034063-022-400-H09C-LB13
GenBank Accession LN512668 [GenBank]
Sequencing primer gene specific primer, LB13: 5'-CGAAAGTGAGTCTTTCGTTTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K034063-022-400-H09C-LB13
TCTTCCGATTCATTTTATGCTCCGCTCGTCGTAGATTAGAGTTTGTGAATCTCCGTTTTA
AAACGCTTAAGAGTTTTGTCCGCTGTTGTGAACTGTTAAATCTGAGTGTTTGCCTTTAGT
AGATTCAAAATCTGTAGATTACTAGAAATATTTTGCCGTATCATAGTATGTGATTCTTCA
TATGCTATCGTTTAATTGTTAAAACATAGTAATTGTCACATTTCTGGGTGTCACGGATTC
ATGATATGTTACTAGCTTGGATTCATTTTGTTGTTCTGATTTGTGAGTTTTATGTTTGGG
TTTGTGAAGGTATGAGATGGGAAGGCAGCCAGCCAACACCAAGCTCTCAAGCAACAAGAC
GGTCAGAAGAATAAGAGTTCGTGGTGGAAATGTTAAGTGGCGTGCGTTGAGGCTCGATAC
TGGTAACTACTCGTGGGGAAGTGAAGCAACTACCCGCAAGACCAGAGTCCTTGATGTGGT
CTACAATGCCTCCAACAATGACTTCATGTACTTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 05-K034063-022-400-H09C-8409
GenBank Accession LN488253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K034063-022-400-H09C-8409
AAGCCATTTACAATTGAATATATCCTGAAGTACATGAAGTCATTGTTGGAGGCATTGTAG
ACCACATCAAGGACTCTGGTCTTGCGGGTAGTTGCTTCACTTCCCCACGAGTAGTTACCA
GTATCGAGCCTCAACGCACGCCACTTAACATTTCCACCACGAACTCTTATTCTTCTGACC
GTCTTGTTGCTTGAGAGCTTGGTGTTGGCTGGCTGCCTTCCCATCTCATACCTTCACAAA
CCCAAACATAAAACTCACAAATCAGAACAACAAAATGAATCCAAGCTAGTAACATATCAT
GAATCCGTGACACCCAGAAATGTGACAATTACTATGTTTTAACAATTAAACGATAGCATA
TGAAGAATCACATACTATGATACGGCAAAATATTTCTAGTAATCTACAGATTTTGAATCT
ACTAAAGGCAAACACTCAGATTTAACAGTTCACAACAGCGGACAAAACTCTTAAGCGTTT
TAAAACGGAGATTCACAAACTCTAATCTACGACGAGCGGAGCATAAAATGAATCGGAAGA
GATCTAAAAAATGAGAAAAACGAAAGATCTACTTTCCGAATTGATTCTATGTTTGATATG
GTTCTTTGTAAATCTCTT

Last Updated on 06.12.2018 16:11