Sequences from confirmation amplicon


Confirmation sequences for line 401C08 and hit At5g23575 are shown.
Line ID 401C08
Locus At5g23575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7948686
Primers used for PCR JY35 - 8474, show primer details
   
Sequence Name 44-K038035-022-401-C08C-8409
GenBank Accession LN505106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K038035-022-401-C08C-8409
TCCGGACATGAGCCATTTACAATTGAATATATCCTGCTTGACGGTGAATCTGACGAACTA
AAGGTAATTCTGGTTATTTATAGTCAATCACCATCTTCTATCCATGAACTTATGATTTGC
TATTAACTTTTGAAACTAGTTGGTGGAGAGACACTATTGATTATTTTCTTTACATATCTC
TTGGTTTCTCCCTGGAGCTGTTTGTAAATATTATTTTGTTGTGTAATTTATTTATTACAG
AAGGTGTTTTTGGAAGGAAATCCCTATCTTTTGGGGATCACGATGTTTGTTTCGATGCTT
CATTCAGTGTTCGACTTCCTGGCATTCAAAAATGGTAAGTAAGACCGAAATCTTTCCTAA
CAATTGTAGGAATGATTGTTGAAATTTTACTAATTTTTGGCCTACCCTGCAGATATCCAA
TTCTGGAACAAAAATAAATCTATGGAAGGGCTGTCTGCAAAGTCTGTTGTACTGAACTTT
ATCTGTCAGTTTGTCATCTTCCTCTACTTGCTTGACAACGACACTTCATGGATGATACTG
GCTAGTTCCGGAGTCGGTGTCTGCATTGAGTTCTGGAAAATAGGGAAAGCCATGCGCATA
GAGGTACTTATAAGTTCTTATATATACATTTCTAATGTTGTTGCTT
   
Sequence Name 04-K038035-022-401-C08C-JY35
GenBank Accession LN487831 [GenBank]
Sequencing primer gene specific primer, JY35: 5'-GTCTACATGCAAAGCAACAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K038035-022-401-C08C-JY35
TAGACTTATAAGTACCTCTATGCGCATGGCTTTCCCTATTTTCCAGAACTCAATGCAGAC
ACCGACTCCGGAACTAGCCAGTATCATCCATGAAGTGTCGTTGTCAAGCAAGTAGAGGAA
GATGACAAACTGACAGATAAAGTTCAGTACAACAGACTTTGCAGACAGCCCTTCCATAGA
TTTATTTTTGTTCCAGAATTGGATATCTGCAGGGTAGGCCAAAAATTAGTAAAATTTCAA
CAATCATTCCTACAATTGTTAGGAAAGATTTCGGTCTTACTTACCATTTTTGAATGCCAG
GAAGTCGAACACTGAATGAAGCATCGAAACAAACATCGTGATCCCCAAAAGATAGGGATT
TCCTTCCAAAAACACCTTCTGTAATAAATAAATTACACAACAAAATAATATTTACAAACA
GCTCCAGGGAGAAACCAAGAGATATGTAAAGAAAATAATCAATAGTGTCTCTCCACCAAC
TAGTTTCAAAAGTTAATAGCAAATCATAAGTTCATGGATAGAAGATGGTGATTGACTATA
AATAACCAGAATTACCTTTAGTTCGTCAGATTCACCGTCAAGCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
C

Last Updated on 06.12.2018 16:11