Sequences from confirmation amplicon


Confirmation sequences for line 401D10 and hit At3g16870 are shown.
Line ID 401D10
Locus At3g16870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5763767
Primers used for PCR 47vd - 8474, show primer details
   
Sequence Name 33-K117737-0022-401-D10C-47vd
GenBank Accession LN501167 [GenBank]
Sequencing primer gene specific primer, 47vd: 5'-AATTTAGTGACCGGAAAGATCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117737-0022-401-D10C-47vd
ATTTTTCTATTTCTTTGGTTTGTCTTTTGCTATGTAGTCATTGTGCAATGCTTGTGGGAT
CAAGAGTAGGAAGAAGAGACAAGCAGCACTTGGTATGAGATCAGAGGAGAAGAAGAAGAA
CAGAAAAAGCAATTGCAATAATGATCTAAACCTCGACCATCGAAACGCCAAGAAATACAA
AATCAACATAGTTGATGATGGCAAGATCGACATCGATGATGATCCGAAAATTTGCAATAA
CAAGCGTAGTAGTAGTAGTAGCAGTAACAAAGGAGTGAGTAAGTTTTTGGATTTAGGGTT
TAAAGTACCGGTGATGAAGAGATCAGCGGTTGAGAAGAAGAGGTTATGGAGAAAACTCGG
TGAGGAAGAAAGAGCTGCTGTGCTTCTCATGGCTCTCTCTTGTAGCCCAATGGATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CACGNTTATT
   
Sequence Name 01-K117736-0022-401-D10C-8409
GenBank Accession LN486758 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117736-0022-401-D10C-8409
TCGGACATGACCATTTACATTGAATATCCATTGGGCTACAAGAGAGAGCCATGAGAAGCA
CAGCAGCTCTTTCTTCCTCACCGAGTTTTCTCCATAACCTCTTCTTCTCAACCGCTGATC
TCTTCATCACCGGTACTTTAAACCCTAAATCCAAAAACTTACTCACTCCTTTGTTACTGC
TACTACTACTACTACGCTTGTTATTGCAAATTTTCGGATCATCATCGATGTCGATCTTGC
CATCATCAACTATGTTGATTTTGTATTTCTTGGCGTTTCGATGGTCGAGGTTTAGATCAT
TATTGCAATTGCTTTTTCTGTTCTTCTTCTTCTCCTCTGATCTCATACCAAGTGCTGCTT
GTCTCTTCTTCCTACTCTTGATCCCACAAGCATTGCACAATGACTACATAGCAAAAGACA
AACCAAAGAAATAAGAAAAATTTGAACTTCAGAGCAATATGAGATCAGATCTTTCCGGTC
ACTAAATT

Last Updated on 06.12.2018 16:11