Sequences from confirmation amplicon


Confirmation sequences for line 401D12 and hit At2g39480 are shown.
Line ID 401D12
Locus At2g39480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16482089
Primers used for PCR JV62 - 8474, show primer details
   
Sequence Name 07-K101324-0022-401-D12C-8409
GenBank Accession LN489203 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K101324-0022-401-D12C-8409
TCGGACTGAGCCTTTACAATTGAATATATCCTGATATCAGGAAGAATTGCGGCGTATAGG
CTTTTTGAGATGATAAGTCGTTCATCCTCTGGGACTAATCAAGAGGGAATTATTTTGTCT
GCTGTCCAAGGAAATATTGAGTTTCGGAATGTATATTTCAGCTATTTGTCACGACCTGAA
ATTCCAATCTTGAGTGGATTTTACCTTACTGTGCCGGCTAAAAAAGCTGTTGCACTTGTT
GGTAGAAATGGTTCTGGAAAAAGCAGTATCATTCCTCTAATGGAACGGTTTTATGATCCT
ACACTAGGTACTATGATGTGTAATTTAAGATGTATCAGTTTTTCTGAATAATCCCCTTTG
GATACTCACCTGCGAAGGATACTATTAGTAACTGATTAGATCTGTGGTTTTCTCTTATAC
AGGAGAAGTTCTACTTGATGGAGAAAATATTAAAAATCTAAAATTAGAGTGGCTGAGAAG
CCAAATAGGCCTGGTGACACAGGAGCCTGCTTTGCTTAGTCTGAGCATAAGAGAGAATAT
TGCATACGGCCGAGATGCTACTCTGGATCAGATAGAGGAAGCAGCTAAAAAAGCACATGC
ACACACATTTATC
   
Sequence Name 07-K101341-0022-401-D12C-JV62
GenBank Accession LN489204 [GenBank]
Sequencing primer gene specific primer, JV62: 5'-AAGTGAGCTGATAAATGTGTGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K101341-0022-401-D12C-JV62
GATCAGAGTAGCATCTCGGCCGTATGCATATTCTCTCTTATGCTCAGACTAAGCAAAGCA
GGCTCCTGTGTCACCAGGCCTATTTGGCTTCTCAGCCACTCTAATTTTAGATTTTTAATA
TTTTCTCCATCAAGTAGAACTTCTCCTGTATAAGAGAAAACCACAGATCTAATCAGTTAC
TAATAGTATCCTTCGCAGGTGAGTATCCAAAGGGGATTATTCAGAAAAACTGATACATCT
TAAATTACACATCATAGTACCTAGTGTAGGATCATAAAACCGTTCCATTAGAGGAATGAT
ACTGCTTTTTCCAGAACCATTTCTACCAACAAGTGCAACAGCTTTTTTAGCCGGCACAGT
AAGGTAAAATCCACTCAAGATTGGAATTTCAGGTCGTGACAAATAGCTGAAATATACATT
CCGAAACTCAATATTTCCTTGGACAGCAGACAAAATAATTCCCTCTTGATTAGTCCCAGA
GGATGAACGACTTATCATCTCANAAAGCCTATACGCCGCANTTCTTCCTGATATCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
A

Last Updated on 06.12.2018 16:11