Sequences from confirmation amplicon


Confirmation sequences for line 402D03 and hit At2g04390 are shown.
Line ID 402D03
Locus At2g04390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1528314
Primers used for PCR CB57 - 8474, show primer details
   
Sequence Name 16-K106136-0022-402-D03C-8409
GenBank Accession LN493437 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106136-0022-402-D03C-8409
CGGACTGAGCCTTTACATTGAAATATTCCGCCGCCGAAAGCAGGGATTGGTGCCATATCT
TGTTGTGGGTCGACTGCAGAGATGCCGAGAGTGTCAGACATTCCTAGAGAAGCAAGCATC
TCAAGAGTCTCTTTGTCGACCTTGATCTCATCAGTCTTGATAGCAGACTCATCGGGAACA
AAGTCCATACGGCGTTCACGCTCTTCTTCTTGAAGCTTGAGTGAGATTCCACGGACTGGT
CCCTTCTGGATACGTTTCATCAAGTGGGTGGAGAATCCAGCAATCTTGTTGCGGAGTCTC
TTTGATGGGATGATGGCAACCTCTTCAAGGATCTTCTTGTTAGTGTGAAAGTCAAGAGTC
ATGCGAGAGTAGTACTTCTCAATGACTTGACGAGAAGATTTCTTCACGGTCTTGGTTCTA
ACACGCCCCATCTTTCAGTTACTTACCTGATGAGAAGAGAAATTCCATGTCTTATTAAGG
ATTCAAAATCAAGAAACAACCAAGTATAGATGATTTATGTCTTAAACTTCAACATTCAGG
TTGAACACTAAACAAACACAGGAACCACATGTAAATGCAATGTCAAATATTGTCAAGAGA
ATTGAAAATGAGTCAAGTAATATACATCAACATAACGCTCAACTTGAAAGAAGTCTA
   
Sequence Name 16-K106135-0022-402-D03C-CB57
GenBank Accession LN493436 [GenBank]
Sequencing primer gene specific primer, CB57: 5'-CTAGACCTCTTTCAAGTTGAGCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K106135-0022-402-D03C-CB57
TTTTAATTCTCTTGACATATTTGACATTGCATTTACATGTGGTTCCTGTGTTTGTTTAGT
GTTCAACCTGAATGTTGAAGTTTAAGACATAAATCATCTATACTTGGTTGTTTCTTGATT
TTGAATCCTTAATAAGACATGGAATTTCTCTTCTCATCAGGTAAGTAACTGAAAGATGGG
GCGTGTTAGAACCAAGACCGTGAAGAAATCTTCTCGTCAAGTCATTGAGAAGTACTACTC
TCGCATGACTCTTGACTTTCACACTAACAAGAAGATCCTTGAAGAGGTTGCCATCATCCC
ATCAAAGAGACTCCGCAACAAGATTGCTGGATTCTCCACCCACTTGATGAAACGTATCCA
GAAGGGACCAGTCCGTGGAATCTCACTCAAGCTTCAAGAAGAAGAGCGTGAACGCCGTAT
GGACTTTGTTCCCGATGAGTCTGCTATCAAGACTGATGAGATCAAGGTCGACAAAGAGAC
TCTTGAGATGCTTGCTTCTCTAGGAATGTCTGACACTCTCGGCATCTCTGCAGTCGACCC
ACAACAAGCTATGGCACCAATCCCTGCTTTCGGCGGCGGAATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11