Sequences from confirmation amplicon


Confirmation sequences for line 403D11 and hit At3g58110 are shown.
Line ID 403D11
Locus At3g58110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21518197
Primers used for PCR 13wm - 8474, show primer details
   
Sequence Name 77-K118585-0022-403-D11C-13wm
GenBank Accession LN513610 [GenBank]
Sequencing primer gene specific primer, 13wm: 5'-GCAAATGACAATCTAGCTTTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118585-0022-403-D11C-13wm
GTCACTGGGGGTGGTTTTTCATCCCAGAGGGTTCTTCGGTCCTCAACCTCTTATTAATGG
GGTTATGAGAATGATAAGTAATACCATTCTCGTGTTCAATCTCCCTCTTGTTATTGCCAT
TCCCGAACAGAGAAGAACTACCTGAACCCATAGCCATATGCATCTCACCCCTAGAAGCAA
GGAAATCACCACCAATTGAATTGCCATGAATTTGTAACCCAGAGTTGTAACCTAACGGAG
ATGCATCTCCCAACATGAGATTCCCTTGGCCAACTCCCTGGAGGGAATCACCATTTGGAA
AAAAAGGCAATGAATCAACTACATGTAAATGGCTATATTCAATTGTAAATGGCTTGTAAA
TGGCTTACATAATTCACTCAAATGCTAGCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 47-K118584-0022-403-D11C-8409
GenBank Accession LN506339 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118584-0022-403-D11C-8409
TTGAATATATCCTGCTAGCATTTGAGTGAATTATGTAAGCCATTTACAAGCCATTTACAA
TTGAATATAGCCATTTACATGTAGTTGATTCATTGCCTTTTTTTCCAAATGGTGATTCCC
TCCAGGGAGTTGGCCAAGGGAATCTCATGTTGGGAGATGCATCTCCGTTAGGTTACAACT
CTGGGTTACAAATTCATGGCAATTCAATTGGTGGTGATTTCCTTGCTTCTAGGGGTGAGA
TGCATATGGCTATGGGTTCAGGTAGTTCTTCTCTGTTCGGGAATGGCAATAACAAGAGGG
AGATTGAACACGAGAATGGTATTACTTATCATTCTCATAACCCCATTAATAAGAGGTTGA
GGACCGAAGAACCCTCTTGGGATGAAAAACCACCCCCAGTTGACATGTGTTTGGATCAGA
TGGCGTACTGGGCGGAAAAAGCTAGATTGTCATTTGC

Last Updated on 06.12.2018 16:11