Sequences from confirmation amplicon


Confirmation sequences for line 403F05 and hit At1g55030 are shown.
Line ID 403F05
Locus At1g55030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20531347
Primers used for PCR 32xe - 8474, show primer details
   
Sequence Name 54-K119004-0022-403-F05C-32xe
GenBank Accession LN508643 [GenBank]
Sequencing primer gene specific primer, 32xe: 5'-GATGATACCGGTAGGAAACGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K119004-0022-403-F05C-32xe
CCGAAGCTTCTCGTCGATTATCTCAGAGACATCAGTAATATTTACCTCTATCAGCTCCGA
TACGTTCTCAACCATAAAAGACTCAAAAGCCTTAGACCCTTCAAGTTTCAAGTATTTCAA
AGAAGGAGTATTTATGGTATATCCACAATGTTGTGGTGTACCACTGCCATTATGAATGGC
TAGTCTTTGTAAAGATGGCACTGCAATAGTGAAAGTTTTCACATTACTACTTGAATTTCT
ACGCATTACCAAATCTTGAAGATTAGGACAGCCAGATAAAAGGTTAATAACTGATTCATC
GTCTTTGAAGTCCACAGCTAGAAGATAAAGGGTTTTAAGGGATTTCAGATAAACCGAAGG
AGGGACATCCATAACAACATGTAAATGGCTATATACATGTAAATGTAAGGGATTTCCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGATTATTA
   
Sequence Name 37-K119003-0022-403-F05C-8409
GenBank Accession LN502798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K119003-0022-403-F05C-8409
CATTTCATTGAATATATCCTGGAAATCCCTTACATTTACATGTATATAGCCATTTACATG
TTGTTATGGATGTCCCTCCTTCGGTTTATCTGAAATCCCTTAAAACCCTTTATCTTCTAG
CTGTGGACTTCAAAGACGATGAATCAGTTATTAACCTTTTATCTGGCTGTCCTAATCTTC
AAGATTTGGTAATGCGTAGAAATTCAAGTAGTAATGTGAAAACTTTCACTATTGCAGTGC
CATCTTTACAAAGACTAGCCATTCATAATGGCAGTGGTACACCACAACATTGTGGATATA
CCATAAATACTCCTTCTTTGAAATACTTGAAACTTGAAGGGTCTAAGGCTTTTGAGTCTT
TTATGGTTGAGAACGTATCGGAGCTGATAGAGGTAAATATTACTGATGTCTCTGAGATAA
TCGACGAGAAGCTTCGGGTATTTCTTACTTCAGTTACACGTCTTTCCTTGGCTTTATCAC
CATCGGTGTTTACGTTTCCTACCGGTATCATC

Last Updated on 06.12.2018 16:11