Sequences from confirmation amplicon


Confirmation sequences for line 403F05 and hit At5g44620 are shown.
Line ID 403F05
Locus At5g44620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17999048
Primers used for PCR 62rq - 8474, show primer details
   
Sequence Name 64-K116102-0022-403-F05C-62rq
GenBank Accession LN511274 [GenBank]
Sequencing primer gene specific primer, 62rq: 5'-CAAGGACCAACTTGACTATGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K116102-0022-403-F05C-62rq
ACGGCGGACTCCACCGTAGCCCCTAAGACCTTGGGGCCTTCCCATTATCGGAAACCTTCC
ATTCCTCCAACCGGAGCTTCACACCTACTTCCAAGGGCTGGCTAAAAAGCACGGTCCCAT
TTTCAAACTCTGGCTCGGGGCCAAGCTCACAATCGTGGTCACCTCTACTGAAGTAGCGCA
AGAAATTCTCAAAACGAATGACATCATCTTCACGAACCACGATGTCCCTGCTGTGAGCCC
TGTTAACACATATGGAGGTACGGAGATCATTTGGTCGCCGTATGGACCAAAATGGCGGAT
GCTGAGGAAGCTATGTGTTAATATGATACTGAGAAACGCCATGTTGGATTCCTCCACTGA
CACTCCGTCGCCTAAATACCAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAACAATGAGTATGTGGGTCAATAGAGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 46-K116101-0022-403-F05C-8409
GenBank Accession LN505967 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K116101-0022-403-F05C-8409
ATTTACATTGAATATTTGGTATTTCGGCGACGGAGGTCAGTGGAGGAATCCAACATGGGC
GTTTCTCAGTATCCTATTAACACATAGCTTCCTCAGCATCCGCCACTTTGGTCCATACGG
CGACCAAATGATCTCCGTACCACCATACGTGTTAACAGGGCCCACAGCAGGGACATCGTG
GTTCGCGAAGATGATGTCATTCGTTTTGAGAATCTCTTGCGCTACTTCAGAAGAGGTGAC
CACGATTGTGAGCTTGGCCCCGAGCCAGAGTTTGAAAATGGGACCGTGCTTTTTAGCCAG
CCCTTGGAAGTAGGTGTGAAGCTCCGGTTGGAGGAATGGAAGGTTTCCGATAATGGGAAG
GCCCCAAGGTCCCGGCGGCAACGGTGGAGACCGCCGTTTGCATTTGGCGTAGAGCCAAAG
ACACCAACAAAGAGTTGATATAACTATCACAGTTAAACCATAGTCAATGGGGGTCCTTG

Last Updated on 06.12.2018 16:11