Sequences from confirmation amplicon


Confirmation sequences for line 403H03 and hit At5g01630 are shown.
Line ID 403H03
Locus At5g01630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:240485
Primers used for PCR 08im - 8474, show primer details
   
Sequence Name 48-K112831-0022-403-H03C-08im
GenBank Accession LN506547 [GenBank]
Sequencing primer gene specific primer, 08im: 5'-AACTCAAGAAACCTGGAAGTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112831-0022-403-H03C-08im
TGTAATGCGCACACAGCTGGATATAAGACTTAAAAAAAGAGGAGATAAGAACACACCTGA
GTCAATTATCATATCACTTCCTAGAATAGACAAAGGTTTGGCAATTGAAGACTCTTTCAA
AGGGACAGTTTTCCCCAAGGCCGTCCTGAACATTGGCATAGTTTCAGCTGTATCCACTTG
TCTCGTTTGGGGAATTGAGCAATTTGTGTTCTGCAAATCTAAAAGTGTAGCCAAGAACAT
GGAACATTCAAAAGATTTTCAGGAACACATATTCACCAACATTAAGTAACAATTCAAGCG
AATTCAGAAACAAATGACTAGTCAATGTACGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACAGTTATT
   
Sequence Name 19-K112829-0022-403-H03C-8409
GenBank Accession LN494833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112829-0022-403-H03C-8409
GTTTCTGATTCGCTTGAATTGTTACTTAATGTTGGTGAATATGTGTTCCTGAAAATCTTT
TGAATGTTCCATGTTCTTGGCTACACTTTTAGATTTGCAGAACACAAATTGCTCAATTCC
CCAAACGAGACAAGTGGATACAGCTGAAACTATGCCAATGTTCAGGACGGCCTTGGGGAA
AACTGTCCCTTTGAAAGAGTCTTCAATTGCCAAACCTTTGTCTATTCTAGGAAGTGATAT
GATAATTGACTCAGGTGTGTTCTTATCTCCTCTTTTTTTAAGTCTTATATCCAGCTGTGT
GCGCATTTACATATGAACTTGTTGTTTGTGCTGCTACTCCGTTCAACTTCCAGGTTTCTT
GAGT

Last Updated on 06.12.2018 16:11