Genotyping and primer details

Genotyping and primer details for 403H03 - At5g01630
Line ID 403H03
Locus At5g01630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:240485 of line 403H03 Chr5:240485
Insertion allele amplicon 8474 - 08im
Locus specific primer 08im: 5'-AACTCAAGAAACCTGGAAGTTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:240120
wt amplicon (over InsPos) 64rd - 08im
wt amplicon start Chr4:10170
wt amplicon end Chr5:240120
wt amplicon size 597 bp
Locus specific primer 08im: 5'-AACTCAAGAAACCTGGAAGTTGAA-3'
2nd locus specific primer 64rd: 5'-GTCATGTGACTCAAAGGAGATTGA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11