Sequences from confirmation amplicon


Confirmation sequences for line 405B10 and hit At1g08130 are shown.
Line ID 405B10
Locus At1g08130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2548020
Primers used for PCR PF71 - 8474, show primer details
   
Sequence Name 13-K103074-0022-405-B10C-PF71
GenBank Accession LN491994 [GenBank]
Sequencing primer gene specific primer, PF71: 5'-CACAAATTAACAGGCGGATTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103074-0022-405-B10C-PF71
GAAATATGTGTTTTATTTTAATTCTACACTAAGATTTGTATCCTAATTGGCCAATTAAAC
AAGATAAAGATTAAAAGTTGAGGAACTCGTAAACAGCCCTTCACGCGCTTAATTTTTTAA
TTTTACGGAAAACAGCAATTTCGCATGGATTTCGGATAGGTGGCGTCTACGCGCTCATTG
TGCGAATGAGTAAGATACTTAGATTTCACAGTTCGATAACCATACGCGTCACGTTTGGGC
TGACATTATACTGTCTATTGGGCTATAACGATATTTTTTCCATATCATAGACCCACATAA
AATAAAAAGGCCCAATAACAAAAATTTAAAACTAGGGCACAGTTTAGTGTATGAGTATCC
ATTAGTGATAGCATTTGATGTAATTTATAAAATATTATATTTACAATAACCTTTGAAATA
ATCATCACAATTTTGTCCAAATTTGAAGTTTTCAAAAAAATAAAATTTGATCATTGGGAT
CTATTAAAGATTTGGACTAACCCTAATTAGTAATTACAGGTAGAAACTCTGTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAAATA
   
Sequence Name 14-K103074-0022-405-B10C-8409
GenBank Accession LN492464 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103074-0022-405-B10C-8409
GCCATTTACACANAGTTTCTACCTGTAATTACTAATTAGGGTTAGTCCAAATCTTTAATA
GATCCCAATGATCAAATTTTATTTTTTTGAAAACTTCAAATTTGGACAAAATTGTGATGA
TTATTTCAAAGGTTATTGTAAATATAATATTTTATAAATTACATCAAATGCTATCACTAA
TGGATACTCATACACTAAACTGTGCCCTAGTTTTAAATTTTTGTTATTGGGCCTTTTTAT
TTTATGTGGGTCTATGATATGGAAAAAATATCGTTATAGCCCAATAGACAGTATAATGTC
AGCCCAAACGTGACGCGTATGGTTATCGAACTGTGAAATCTAAGTATCTTACTCATTCGC
ACAATGAGCGCGTAGACGCCACCTATCCGAAATCCATGCGAAATTGCTGTTTTCCGTAAA
ATTAAAAAATTAAGCGCGTGAAGGGCTGTTTACGAGTTCCTCAACTTTTAATCTTTATCT
TGTTTAATTGGCCAATTAGGATACAAATCTTAGTGTAGAATTAAAATAAAACACATATTT
CAATAATTTAGTGGTTGCACATGCACCCTGTATTTCTTCACTAAATCCGCCTGTAAATTT
GTG

Last Updated on 06.12.2018 16:11