Sequences from confirmation amplicon


Confirmation sequences for line 405B10 and hit At3g55550 are shown.
Line ID 405B10
Locus At3g55550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20601356
Primers used for PCR 25bc - 8474, show primer details
   
Sequence Name 59-K107060-0022-405-B10C-8409
GenBank Accession LN510014 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K107060-0022-405-B10C-8409
GCCATTTACAATTGAATATATCCTAAGATATCCGAACATACATTTCATCTCCCAAAACAG
AGGATAGGTCAACATCGTAAGAGAGAAGAGACAACTTTGGTTTCTCTGAGAATGGAGAAA
GTTTAACATCTAACCTTTTCTTGTTTGAATCGTAATCAATCCAAGCTTGAATCACTCTAC
CACCATCAAGGAAAAGCTCCTTTTTGGTTGAGTTAGCGAGGAAATAGCCAGCAGGAGTCG
AAATACTCGATTCCATACTGTTTATATCGATTCCTACATGGTTATCATTAATGTCCTCAA
ACTCCAAATCCCTGACAGTATCGAATTCCACAGCGAAGAAGTGGCTAGAGAAGTTAACTC
TGCTTGAATTCAACAGGCCTAAGTACTGGCTAGGAAGAGAACCTCTGAGATCTGGAGTTG
GAGTGATGGCGAAGGCAAGTCCGTGGCCACCAAGAGTAACAAACTCTGGAACCATGGCGA
TTGCAAAAGACGTGGAGAAAGACAGAGCTCGGTTTACACCGATTGGCTTGAACCGTATTG
GTAAGGAGTAGAAAGCGTGACCAATCACGCGCTGTGTCTCTGTTGTGAGCCTGATTGCTC
CAGTGGGGGCGATCTCTGCAACTCCGTTTAACGTTAAATTTGGTGAGGCTTTTTTGAAGC
CAATGAAGGAG
   
Sequence Name 51-K107061-0022-405-B10C-25bc
GenBank Accession LN507518 [GenBank]
Sequencing primer gene specific primer, 25bc: 5'-AATTCAAGACTTCTCCTTCATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K107061-0022-405-B10C-25bc
ATTTACGTTAACGGAGTTGCAGAGATCGCCCCCACTGGAGCAATCAGGCTCACAACAGAG
ACACAGCGCGTGATTGGTCACGCTTTCTACTCCTTACCAATACGGTTCAAGCCAATCGGT
GTAAACCGAGCTCTGTCTTTCTCCACGTCTTTTGCAATCGCCATGGTTCCAGAGTTTGTT
ACTCTTGGTGGCCACGGACTTGCCTTCGCCATCACTCCAACTCCAGATCTCAGAGGTTCT
CTTCCTAGCCAGTACTTAGGCCTGTTGAATTCAAGCAGAGTTAACTTCTCTAGCCACTTC
TTCGCTGTGGAATTCGATACTGTCAGGGATTTGGAGTTTGAGGACATTAATGATAACCAT
GTAGGAATCGATATAAACAGTATGGAATCGAGTATTTCGACTCCTGCTGGCTATTTCCTC
GCTAACTCAACCAAAAAGGAGCTTTTCCTTGATGGTGGTAGAGTGATTCAAGCTTGGATT
GATTACGATTCAAACAAGAAAAGGTTAGATGTTAAACTTTCTCCATTCTCAGAGAAACCA
AAGTTGTCTCTTCTCTCTTACGATGTTGACCTATCCTCTGTTTTGGGAGATGAAATGTAT
GTTCGGATATCTTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGT

Last Updated on 06.12.2018 16:11