Sequences from confirmation amplicon


Confirmation sequences for line 405D02 and hit At1g77630 are shown.
Line ID 405D02
Locus At1g77630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29175328
Primers used for PCR 29cp - 8474, show primer details
   
Sequence Name 90-K108704-0022-405-D02C-29cp
GenBank Accession LN515048 [GenBank]
Sequencing primer gene specific primer, 29cp: 5'-ACTTGTAGTTACAACGGTTTCGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K108704-0022-405-D02C-29cp
TCTTTCCTTATCACATTTCCCCTGCTAATGAATTTAACAACCTATATAACCAATCTCCCT
CCGTCTCTCTTTGTTTGTTTTTCAGGTTGTCCATGTCTCTTCAACCTCGATGCCCTGGTA
AGTATACTGTCTCATGCGTTATGATAAAACACAATGAGTGAAATCTTGATCTCCAGCTGA
AATGCAATTGGTAAAAACAATTTGCAGGACCGCAACAATTAGCACCGCTTATAGCCCCGC
CTGATAATGTTCCAAAGGAACTGATGTATCTACCTTCACCTTCACCTTCGCCATCACCAG
AGTTTGATGATATTGCTGGTGGAGGATCATCTATAGCCGCAGTCCCCGCGGCTTCACCTG
GAGGAGCTACGGTTTCTTCAAGCAATTCAATCCCGGGTAATCCAGCTAATGGTCCAGGCG
GAAGTATTTCTATAGCTTCTTGTCCATTGATCCATTTACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTA
TTA
   
Sequence Name 06-K108703-0022-405-D02C-8409
GenBank Accession LN488848 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K108703-0022-405-D02C-8409
ATTTACATTGAATTATCCTGTAAATGGATCAATGGACAAGAAGCTATAGAAATACTTCCG
CCTGGACCATTAGCTGGATTACCCGGGATTGAATTGCTTGAAGAAACCGTAGCTCCTCCA
GGTGAAGCCGCGGGGACTGCGGCTATAGATGATCCTCCACCAGCAATATCATCAAACTCT
GGTGATGGCGAAGGTGAAGGTGAAGGTAGATACATCAGTTCCTTTGGAACATTATCAGGC
GGGGCTATAAGCGGTGCTAATTGTTGCGGTCCTGCAAATTGTTTTTACCAATTGCATTTC
AGCTGGAGATCAAGATTTCACTCATTGTGTTTTATCATAACGCATGAGACAGTATACTTA
CCAGGGCATCGAGGTTGAAGAGACATGGACAACCTGAAAAACAAACAAAGAGAGACGGAG
GGAGATTGGTTATATAGGTTGTTAAATTCATTAGCAGGGGAAATGTGATAAGGAAAGACT
CATTACGTGGTCAAGATAGTGCCGCTAGCGAAACCGTTGTACCTACAAG

Last Updated on 06.12.2018 16:11