Sequences from confirmation amplicon


Confirmation sequences for line 405E02 and hit At1g78890 are shown.
Line ID 405E02
Locus At1g78890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29657201
Primers used for PCR CB65 - 8474, show primer details
   
Sequence Name 22-K106138-0022-405-E02C-8409
GenBank Accession LN496121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106138-0022-405-E02C-8409
CGGACTGAGCCATTTACATTGAACCTGTAAAGAATTTTCACAACTATATAACGATAAGCC
AAGAATTGCCTAAGTGCATCTAAGAATGTAGCAATACCTATGCAGATTATAGATACTGCA
AATGTAAAAACCAAAGCTTTTTCAAAACCGAAACACATACGATCAGACAATTAGAGTTAA
TCATATAAAAACCACCTTTGATGAAGTAATCAGAGCAAGGCCATCCTCGAACCGATGAGA
TTGAAATAGATTCTTCATCAGAGATCGGATTGGCCAGCTTCTCAACGAGCTTAGCGATCC
CATGAGACTGGGATCTAGGAGGTGGAACCCAAAAGGAAGCACCGGGGACAAAAGGGAGCC
AATCAGGTGCGGATCGGCGTACCATAATCCGGTGGACAGCATCTTCCAACTAATTGACA
   
Sequence Name 22-K106137-0022-405-E02C-CB65
GenBank Accession LN496120 [GenBank]
Sequencing primer gene specific primer, CB65: 5'-TTGTCAATAAGTTGGAAGATGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K106137-0022-405-E02C-CB65
TGATTGGCTCCCTTTTGTCCACGGTGCTTCCTTTTGGGTTCCACCTCCTAGATCCCAGTC
TCATGGGATCGCTAAGCTCGTTGAGAAGCTGGCCAATCCGATCTCTGATGAAGAATCTAT
TTCAATCTCATCGGTTCGAGGATGGCCTTGCTCTGATTACTTCATCAAAGGTGGTTTTTA
TATGATTAACTCTAATTGTCTGATCGTATGTGTTTCGGTTTTGAAAAAGCTTTGGTTTTT
ACATTTGCAGTATCTATAATCTGCATAGGTATTGCTACATTCTTAGATGCACTTAGGCAA
TTCTTGGCTTATCGTTATATAGTTGTTGAAAATTCTTTACAGGTTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR CB64 - 8474, show primer details
   
Sequence Name 23-K106138-0022-405-E02C-OriL
GenBank Accession LN496563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106138-0022-405-E02C-OriL
CCATTTACATTGATCCATGTTATTGCTGATCAGTAATAACATAGAGAGTCTTTCCTTTAT
AGGGTTGAAAAGATTGTAGTGTTTATTAATCTTGTTGCTTTTGCTTCTTGTCGAATAATA
ATCACTCTGTTTTATAATAGATTGTGGTGTTTGCATTCTTCAGTGGCAAATGTTGCAGCG
TCAAAATATGTTTTTTTGTTAGGTACAGTGTTTCTTTAACGTTTGGTCTTTTGAGCGTTT
CTTAACACTTGGTCTGTTTTTGATTATAGGTGTAAAGCCTCAATCAGTTGAGACGGAGAT
GACTTCAAATACTGCATTCNACTCCGA
   
Sequence Name 23-K106137-0022-405-E02C-CB64
GenBank Accession LN496562 [GenBank]
Sequencing primer gene specific primer, CB64: 5'-CTCGGAGTGATATGCAGTATTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106137-0022-405-E02C-CB64
CTTTCCCTATAATCAAAACAGACCAAGTGTTAAGAAACGCTCAAAAGACCAAACGTTAAA
GAAACACTGTACCTAACAAAAAAACATATTTTGACGCTGCAACATTTGCCACTGAAGAAT
GCAAACACCACAATCTATTATAAAACAGAGTGATTATTATTCGACAAGAAGCAAAAGCAA
CAAGATTAATAAACACTACAATCTTTTCAACCCTATAAAGGAAAGACTCTCTATGTTATT
ACTGATCAGCAATAACATGGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11