Sequences from confirmation amplicon


Confirmation sequences for line 406C03 and hit At1g24320 are shown.
Line ID 406C03
Locus At1g24320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8629215
Primers used for PCR 19vh - 8474, show primer details
   
Sequence Name 59-K118077-0022-406-C03C-19vh
GenBank Accession LN510100 [GenBank]
Sequencing primer gene specific primer, 19vh: 5'-CGACGAACCAATCAACTATAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118077-0022-406-C03C-19vh
TCGTATAATGAGTCCTTACAGGTTTATATGGCCTACATTTTCTTCAACAAGCAAAATGTT
AATAGTTTTGGATAGTGGACACCATTTCTCTGTTGTAGGTCTAAGAGCATAGTTTCCATA
TAATAACATGTTTTGTCTAACACCATTTTTGATTAGCTGGGATTCATGTTATCTTGTGTA
CTTAGCTTCTACGCCTTTAAGCTAAGTTTCCGTCTTTATTTCGTGTAGGTTAACTGTCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCA
   
Sequence Name 34-K118076-0022-406-C03C-8409
GenBank Accession LN501587 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118076-0022-406-C03C-8409
TGACAGTTAACCTACACGAAATAAAGACGGAAACTTAGCTTAAAGGCGTAGAAGCTAAGT
ACACAAGATAACATGAATCCCAGCTAATCAAAAATGGTGTTAGACAAAACATGTTATTAT
ATGGAAACTATGCTCTTAGACCTACAACAGAGAAATGGTGTCCACTATCCAAAACTATTA
ACATTTTGCTTGTTGAGAAAATGTAGGCCATATAAACCTGTAAGACTCATTATACGATTT
TCCATGTTGGACGTTTCTTCTTTTATTCCAGAGACAAAGGCAATGTCTATAGTTGATTGG
GTTCGTCG

Last Updated on 06.12.2018 16:11