Genotyping and primer details


Genotyping and primer details for 406C03 - At1g24320
Line ID 406C03
Locus At1g24320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8629215 of line 406C03 Chr1:8629215
   
Insertion allele amplicon 8474 - 19vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19vh: 5'-CGACGAACCAATCAACTATAGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8629513
   
wt amplicon (over InsPos) 77wb - 19vh
wt amplicon start Chr1:8628651
wt amplicon end Chr1:8629513
wt amplicon size 863 bp
Locus specific primer 19vh: 5'-CGACGAACCAATCAACTATAGACA-3'
2nd locus specific primer 77wb: 5'-CGCCTGAATCCATATAAAAACAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11