Sequences from confirmation amplicon


Confirmation sequences for line 406F06 and hit At2g40250 are shown.
Line ID 406F06
Locus At2g40250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16814153
Primers used for PCR 64qa - 8474, show primer details
   
Sequence Name 43-K115481-0022-406-F06C-64qa
GenBank Accession LN504948 [GenBank]
Sequencing primer gene specific primer, 64qa: 5'-CTCAAACCCACTCTACCAGCTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115481-0022-406-F06C-64qa
GCGTTAGTTTCGCCTCGGCCGGAGGTGGTTTAGATGATCGGACGGCAAAGTCATCATTGA
CTATTACCATGGACAAGCAATGGAGTTACTTCGAAGAGGCGCTGGGTAAAATGAAGAGTT
TGGTTGGAGACTCGGAGACTAATCGGGTGATTAAGAATGCTGTGTTCGTCATTAGTGCGG
GGACTAATGATATGATCTTTAATGTTTACGATCATGTTCTTGGGAGTTTGATCTCCGTTT
CGGATTATCAAGATTCTCTACTAACCAAAGTCGAAGTTTTTGTCCAGGTTAGTTTCAACG
TTTACTTTAAAGTTCATATATATAGTTTTATCGGATTTATTGATATATATATATATATAT
ATATAGTTCATATATACTGTATATCAGAGTTCAAAAATAGTATATTTCCTACGTACATGG
ATCCCACTCATTAAAGTTATTCACTAGAGTAAGACCTGTACGCATTTGCTTATATAAAGA
TTAGATTTTACTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCTATATGGAGAAAAAGAAAGAGTAATTACCATTTTTTTTTCAATTCAAA
AATGTAGATG
   
Sequence Name 15-K115480-0022-406-F06C-8409
GenBank Accession LN493107 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115480-0022-406-F06C-8409
AATCTAATCATTATATAAGCAAATGCGTACAGGTCTTACTCTAGTGAATAACTTTAATGA
GTGGGATCCATGTACGTAGGAAATTTACTATTTTTGAACTTTGATATACAGTATATATGA
ACTATATATATATATATATATATATCAATAAATCCGATAAAACTATATATATGAACTTTA
AAGTAAACGTTGAAACTAACCTGGACAAAAACTTCGACTTTGGTTAGTAGAGAATCTTGA
TAATCCGAAACGGAGATCACACTCCCAAGAACATGATCGTAAACATTAAAGATCATATCA
TTAGTCCCCGCACTAATGACGAACACAGCACTCTTAATCACCCGATTAGTCTCCGAGTCT
CCAACCAAACTCTTCATTTTACCCAGCGCCTCTACAAAGTAACTCCTTTGCTTGTCCATG
GTAATAGTCAATGATGACTTTGCCGTCAGATCATCTAAACCACCTCCCGCCGAGGCGAAA
CTAACGCCTGTAAAAATATCTACGTGTTTGACCGAAGGGTTGAGGTAAGCTGGTAGAGTG

Last Updated on 06.12.2018 16:11