Sequences from confirmation amplicon


Confirmation sequences for line 407B03 and hit At1g71490 are shown.
Line ID 407B03
Locus At1g71490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26934164
Primers used for PCR 16de - 8474, show primer details
   
Sequence Name 50-K109414-0022-407-B03C-16de
GenBank Accession LN507197 [GenBank]
Sequencing primer gene specific primer, 16de: 5'-TTTAGAAATATGGGTATTGCTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K109414-0022-407-B03C-16de
ATAACTGTTATGCTTCGGAAGGCATGCCGTGTGAAGCGTTTGAGCTTTTCGATAAAATGT
GGTTTTCAGGTGTTGAGGTCAGTGTCATAACTTGGAATATTATTTCTGGAGGTTGTTTGC
AAACAGGAAATTATGTAGGAGCTTTGGGTTTGATCTCTAGGATGAGAAATTTTCCTACGA
GCTTAGATCCTGTGGCTATGATTATTGGTTTGAAGGCTTGCTCTCTCATAGGAGCAATAC
GATTGGGAAAAGAGATTCACGGTCTTGCTATTCACAGTTCTTATGATGGGATTGACAATG
TTCGAAACACGTTGATCACAATGTACTCAAAATGCAAAGACCTTAGGCACGCCTTAATAG
TGTTTCGACAGACTGAAGAAAATAGTTTGTGTACTTGGAACTCTATCATTTCCGGTTATG
CGCAGCTGAACAAATCAGAAGAAGCCTCTCACCTGCTGAGGGAGATGTTGGTTGCTGGCT
TTCAGCCAAATTCCATTACACTAGCAAGCATTCTCCCTCTTTGTGCTCGGATTGCAAACC
TGCAACATGGAAAAGAGTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTC
   
Sequence Name 34-K109413-0022-407-B03C-8409
GenBank Accession LN501450 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K109413-0022-407-B03C-8409
TTGAACTCTTTTCATGTTGCAGGTTTGCAATCCGAGCACCAAGAGGGAGAATGCTTGCTA
GTGTAATGGAATTTGGCTGAAAGCCAGCAACCAACATCTCCCTCAGCAGGTGAGAGGCTT
CTTCTGATTTGTTCAGCTGCGCATAACCGGAAATGATAGAGTTCCAAGTACACAAACTAT
TTTCTTCAGTCTGTCGAAACACTATTAAGGCGTGCCTAAGGTCTTTGCATTTTGAGTACA
TTGTGATCAACGTGTTTCGAACATTGTCAATCCCATCATAAGAACTGTGAATAGCAAGAC
CGTGAATCTCTTTTCCCAATCGTATTGCTCCTATGAGAGAGCAAGCCTTCAAACCAATAA
TCATAGCCACAGGATCTAAGCTCGTAGGAAAATTTCTCATCCTAGAGATCAAACCCAAAG
CTCCTACATAATTTCCTGTTTGCAAACAACCTCCAGAAATAATATTCCAAGTTATGACAC
TGACCTCAACACCTGAAAACCACATTTTATCGAAAAGCTCAAACGCTTCACTCCACATGC
CTTCCGAAGCATAACAGTTTATCACTGCATTCCATGAGACAGCATCCCTTTCAAACATTC
GATCAAACAACCTCCGAGCAATACCCATATTTCTAA

Last Updated on 06.12.2018 16:11