Sequences from confirmation amplicon


Confirmation sequences for line 407C06 and hit At3g56030 are shown.
Line ID 407C06
Locus At3g56030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20791982
Primers used for PCR 13wu - 8474, show primer details
   
Sequence Name 53-K118848-0022-407-C06C-13wu
GenBank Accession LN508311 [GenBank]
Sequencing primer gene specific primer, 13wu: 5'-AATTTGCTGTAAATGTTTGCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118848-0022-407-C06C-13wu
TGTTCTCTTTCTGAAAAAAGCTCGACAATTGTACAATTCATAAATTCATACTTGCACAGC
AATACTTGAACTTGAAGCCACAAGTCAAAACATTCTTAATGGAAACAAAAAAACTCTGCA
TGTGAAAAAAAAAAACGCAAAGAAGCTAACTGTTTACACTTACCTCCTTCAAAGTTCATT
TTTATGTCTGTAACAATGGTGATCTCATTCTTAAAATCAAAATCATGATTGTAACACATT
TTTCTCATGAAATTCACGCAAAGCATCACCCATTCTCAATCCTCTAACACTCATCTCCTT
CAAAACCATCTTAGCTTCTTTAAACCTCTTCCTCTTGATAAGCTTACTAGCCAAACTTCC
CAAATTCTCTTCATCTAATCTCTTATCTTTCCCCGCGTACGCCATCACAAACTCCACACT
TAGCGCATAATACCCACTCTCCACCATTTCTCCTATAACGTGCGCATGCGTGGCGTACAA
TACCGATAACCCTTCTTCCTCCATCCTCCTCAAAATCGCCATCGCCGCTTCAACTCTCCC
TGACTTACACGCGCCTAACACAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAAT
GAGTATGATGGTCAATATGGAAAAAAAAAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTA
   
Sequence Name 40-K118847-0022-407-C06C-8409
GenBank Accession LN503881 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118847-0022-407-C06C-8409
GGCGCGTGTAAGTCAGGGAGAGTTGAAGCGGCGATGGCGATTCTGAGGAGGATGGAGGAA
GAAGGGTTATCGGTATTGTACGCCACGCATGCGCACGTTATAGGAGAAATGGTGGAGAGT
GGCTATTATGCGCTAAGTGTGGAGTTTGTGATGGCGTACGCGGGGAAAGATAAGAGATTA
GATGAAGAGAATTTGGGAAGTTTGGCTAGTAAGCTTATCAAGAGGAAGAGGTTTAAAGAA
GCTAAGATGGTTTTGAAGGAGATGAGTGTTAGAGGATTGAGAATGGGTGATGCTTTGCGT
GAATTTCATGAGAAAAATGTGTTACAATCATGATTTTGATTCTAAGAATGAGATCACCAT
TGTTACAGACATAAAAATGAACTTTGAAGGAGGTAAGTGTAAACAGTTAGCTTCTCTGCG
TTTTTTTTTTTCACATGCAAAGTTTTTTTGTTTCCATTAAAAATGTTTTGACTTGTGGCT
TCAAGTTCAAGTATTGCTGTGCAAGTATGAATTTATGAATTGTACAATTGTCGAGCTTTT
TTCATGAAAGAGAACCCTTCAAATAGATGGTGAACTGTTTTGTTTAATTTACAGAGGAGC
AAACTTT

Last Updated on 06.12.2018 16:11