Sequences from confirmation amplicon


Confirmation sequences for line 407E04 and hit At2g01610 are shown.
Line ID 407E04
Locus At2g01610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:274330
Primers used for PCR AH20 - 8474, show primer details
   
Sequence Name 55-K105395-0022-407-E04C-AH20
GenBank Accession LN508810 [GenBank]
Sequencing primer gene specific primer, AH20: 5'-AAAAATGTAACATGAGAACGACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K105395-0022-407-E04C-AH20
CTCCTCTAAAATCTTACGAAAGAAGAGAACAAAGAAGAAATTTTGAAAATAGTGAAAATG
GTAACCGTAAGCCAAAGTCACACGACGACGTTTCTCTTCTTCACCACATTTCTCTTGATA
TTCGGATCAATCTCAGCCGTCCGTTTGCTTCCACGACCAAACACTACAACAACCAACGAT
CTAGATTTCATCCGAACAAGCTGCAACGCTACTCTATATCCAGACGTCTGCTTCACGTCA
CTCTCCGGCTACGCCTCTGCCGTTCAAGACAGTCCGGCGAGGCTAGCCAAGCTCGCAATC
GGCGTTTCACTTTCACAAGCCAAATCCACTGCGGCTTTTCTCTCCAAACTCTCACGCTCT
GCCGCTAAATACTCCGGTGATGGCCACCAAACAGCTTCCGCCGTAATCCGAGACTGCGTT
TCGAACGTCGAAGACGCGGTGGACGAGATGAGAGGATCTCTCCGTCAACTACGCGACATG
AACGGCAGAGGAGGCGGCACGGCAGCTCGGAGGTCGGTAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 39-K105396-0022-407-E04C-8409
GenBank Accession LN503351 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K105396-0022-407-E04C-8409
TCGGACTGAGCCTTTACATTGAATAATCCTACCGACCTCCGAGCTGCCGTGCCGCCTCCT
CTGCCGTTCATGTCGCGTAGTTGACGGAGAGATCCTCTCATCTCGTCCACCGCGTCTTCG
ACGTTCGAAACGCAGTCTCGGATTACGGCGGAAGCTGTTTGGTGGCCATCACCGGAGTAT
TTAGCGGCAGAGCGTGAGAGTTTGGAGAGAAAAGCCGCAGTGGATTTGGCTTGTGAAAGT
GAAACGCCGATTGCGAGCTTGGCTAGCCTCGCCGGACTGTCTTGAACGGCAGAGGCGTAG
CCGGAGAGTGACGTGAAGCAGACGTCTGGATATAGAGTAGCGTTGCAGCTTGTTCGGATG
AAATCTAGATCGTTGGTTGTTGTAGTGTTTGGTCGTGGAAGCAAACGGACGGCTGAGATT
GATCCGAATATCAAGAGAAATGTGGTGAAGAAGAGAAACGTCGTCGTGTGACTTTGGCTT
ACGGTTACCATTTTCACTATTTTCAAAATTTCTTCTTTGTTCTCTTCTTTCGTAAGATTT
TTAGAGGAGTGATTGGCGGTTTATGTAGGCCTTATAGGGATGGGTCGTTCTCTGTTACCA
TTT

Last Updated on 06.12.2018 16:11