Sequences from confirmation amplicon


Confirmation sequences for line 407F09 and hit At3g23280 are shown.
Line ID 407F09
Locus At3g23280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8322351
Primers used for PCR AC75 - 8474, show primer details
   
Sequence Name 58-K104216-0022-407-F09C-AC75
GenBank Accession LN509694 [GenBank]
Sequencing primer gene specific primer, AC75: 5'-GTTGGTTTGTTCTCCATTTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K104216-0022-407-F09C-AC75
ATTAGGGTTCAAATTGATTTTGTGTGTGTTTTTGGGATTGCAGTGGATGGATAGAGAAGG
AAAAACTCCGTTGATCTTGGCTTGTATGAACTCCGAGCTATTTGATGTTGCTAAGACTTT
GATTGAGTTGGGTTCTAATGTTAATGCTTATCGTCCTGGTATGTCAAAGATCTTAACTTT
TCTTGTAATCCTAGCTTTGAGTAGCTGACTCAATGCTTAGTTAGGATCCGGTGATAATCA
TTGAAATGTAACAACTTGTTAAGTTGAAGTTAGTGGAATGTGTTTGGAGTTTGAGACATT
GTTGATTCTGTTAGAGGTATTTTGAATCAGTTCTGTGATATTAGATGCTTTGGTTTTAGA
GTGTGATTGAGCTAATGTGGTCATATGTGTTTCTCTTCAGGTCGTCATGCTGGGACTCCT
CTGCACCATGCTGCAAAAAGAGGTCTTGAGAACACTGTTAAGTTACTTCTTTCTCACGGT
GGTAAGTGTTTGTCTTCTCATACAACTCGATGAATGTGAGGTCAGTGAATACTTATGGTG
TCTTTATCTCATATGGTGTAATCCCATTTTCTTGTAGCGAATCCACTTGTTCTTAATGAT
GATTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCC
   
Sequence Name 18-K104217-0022-407-F09C-8409
GenBank Accession LN494284 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104217-0022-407-F09C-8409
CCATTTACAATTGAATATATCCTGACAATCATCATTAAGAACAAGTGGATTCGCTACAAG
AAAATGGGATTACACCATATGAGATAAAGACACCATAAGTATTCACTGACCTCACATTCA
TCGAGTTGTATGAGAAGACAAACACTTACCACCGTGAGAAAGAAGTAACTTAACAGTGTT
CTCAAGACCTCTTTTTGCAGCATGGTGCAGAGGAGTCCCAGCATGACGACCTGAAGAGAA
ACACATATGACCACATTAGCTCAATCACACTCTAAAACCAAAGCATCTAATATCACAGAA
CTGATTCAAAATACCTCTAACAGAATCAACAATGTCTCAAACTCCAAACACATTCCACTA
ACTTCAACTTAACAAGTTGTTACATTTCAATGATTATCACCGGATCCTAACTAAGCATTG
AGTCAGCTACTCAAAGCTAGGATTACAAGAAAAGTTAAGATCTTTGACATACCAGGACGA
TAAGCATTAACATTAGAACCCAACTCAATCAAAGTCTTAGCAACATCAAATAGCTCGGAG
TTCATACAAGCCAAGATCAACGGAGTTTTTCCTTCTCTATCCATCCACTGCAATCCCAAA
AACACACACAAAATCAATTTGAACCCTTAATTTCAATCAATTCAAAACAAATTTGATTAA
ACAAAAATGGA

Last Updated on 06.12.2018 16:11