Sequences from confirmation amplicon


Confirmation sequences for line 407F09 and hit At4g23200 are shown.
Line ID 407F09
Locus At4g23200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12146629
Primers used for PCR 46wx - 8474, show primer details
   
Sequence Name 37-K118809-0022-407-F09C-46wx
GenBank Accession LN502794 [GenBank]
Sequencing primer gene specific primer, 46wx: 5'-CCTCCGATAATGTTGTATCGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K118809-0022-407-F09C-46wx
AAGTAGTCATGAGCCACTTAGAGATTACATCATATATGTCTACTCAGAATCTCAGGTTAA
TATGTATATGGTAATGCCACATACCAAAAAGGAAGTAGTCTAGGCTCTTGTTGGGGACAA
ACTCGTAAACAAGTATTTTCTCCTCTGGTTCCAGACAGTACCCAAGAAGCTTAACAAGAT
TCCTATGCTGAAGTTTGGCGACAAGAACAACCTCGTTCTTGAACTCTTGTGCACCTTGTT
CGGATGTTTTCGATAGCCTCTTCACTGCAACTTCTGTTCCATTAACTAACGTGCCCTAAA
AACGGATGATGATATGATCAATTTACCCTAACTCTGTAATGAACCAGGTAGCCATTCCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCTACAAGATTATTA
   
Sequence Name 05-K118808-0022-407-F09C-8409
GenBank Accession LN488569 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118808-0022-407-F09C-8409
TTTACAATTGAATATATCCTGGAATGGCTACCTGGTTCATTACAGAGTTAGGGTAAATTG
ATCATATCATCATCCGTTTTTAGGGCACGTTAGTTAATGGAACAGAAGTTGCAGTGAAGA
GGCTATCGAAAACATCCGAACAAGGTGCACAAGAGTTCAAGAACGAGGTTGTTCTTGTCG
CCAAACTTCAGCATAGGAATCTTGTTAAGCTTCTTGGGTACTGTCTGGAACCAGAGGAGA
AAATACTTGTTTACGAGTTTGTCCCCAACAAGAGCCTAGACTACTTCCTTTTTGGTATGT
GGCATTACCATATACATATTAACCTGAGATTCTGAGTAGACATATATGATTGTAATCTCT
AAGTGGCTCATGACTACTTGTGTACGTGCAGATCCTACAAAGCAAGGGCAGTTAGACTGG
ACAAAACGATACAACGTTATCGGAGG

Last Updated on 06.12.2018 16:11