Sequences from confirmation amplicon


Confirmation sequences for line 407G02 and hit At1g62590 are shown.
Line ID 407G02
Locus At1g62590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23177846
Primers used for PCR CB68 - 8474, show primer details
   
Sequence Name 24-K106138-0022-407-G02C-8409
GenBank Accession LN497000 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106138-0022-407-G02C-8409
CGGATGAGCATTTACAATTGAATATGATAAATGCCCAAAAGGTTTTCAAACAGATGGTTT
CTGATGGTGTACCTCCTGATATAATGACATACAGCATTTTGTTAGATGGACTCTGTAATA
ACGGGAAGCTAGAGAAAGCATTGGAGGTTTTCGACTATATGCAAAAGAGTGAGATAAAAC
TTGATATTTACATATATACTACTATGATTGAAGGGATGTGCAAGGCTGGGAAGGTGGATG
ATGGGTGGGATCTATTTTGTAGCCTCAGCCTTAAAGGAGTGAAGCCTAATGTTGTAACCT
ACAATACCATGATCTCAGGCTTGTGTAGTAAACGCCTATTACAGGAAGCTTATGCCTTGC
TAAAAAAAATGAAAGAAGACGGGCCTCTTCCAAATAGTGGTACATATAATACACTTATCA
GGGCACATCTGAGAGATGGTGACAAAGCAGCATCAGCTGAACTCATCAGAGAAATGAGGA
GTTGCAGGTTTGTTGGAGATGCTTCAACCATTGGCTTGGTCGCTAATATGTTGCATGATG
GGAGATTAGACAAAAGCTTCCTCGATATGCTTTCTTAAAACACTTGCATCATCCCTTGA
   
Sequence Name 24-K106137-0022-407-G02C-CB68
GenBank Accession LN496999 [GenBank]
Sequencing primer gene specific primer, CB68: 5'-CTCAAGGATGATGCAAGTGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106137-0022-407-G02C-CB68
TCTCCATCATGCAACATATTAGCGACCAAGCCAATGGTTGAAGCATCTCCAACAAACCTG
CAACTCCTCATTTCTCTGATGAGTTCAGCTGATGCTGCTTTGTCACCATCTCTCAGATGT
GCCCTGATAAGTGTATTATATGTACCACTATTTGGAAGAGGCCCGTCTTCTTTCATTTTT
TTTAGCAAGGCATAAGCTTCCTGTAATAGGCGTTTACTACACAAGCCTGAGATCATGGTA
TTGTAGGTTACAACATTAGGCTTCACTCCTTTAAGGCTGAGGCTACAAAATAGATCCCAC
CCATCATCCACCTTCCCAGCCTTGCACATCCCTTCAATCATAGTAGTATATATGTAAATA
TCAAGTTTTATCTCACTCTTTTGCATATAGTCGAAAACCTCCAATGCTTTCTCTAGCTTC
CCGTTATTACAGAGTCCATCTAACAAAATGCTGTATGTCATTATATCAGGAGGTACACCA
TCAGAAACCATCTGTTTGAAAACCTTTTGGGCATTTATCATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
Primers used for PCR 46of - 8474, show primer details
   
Sequence Name 43-K114678-0022-407-G02C-46of
GenBank Accession LN504933 [GenBank]
Sequencing primer gene specific primer, 46of: 5'-TCACAAGAAGAGATGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114678-0022-407-G02C-46of
AAGATACACACATCTTATGGCAGAATTAGATTCTCAGGTTCGTACTGTATTTCTTTCCAT
AACATGTATGAAAACATTATCCAAATAGAAAGCATACATTAATTTGAATGAGTTTTTTTG
GTTACAGTTTGTATACAATAACTGGCTTGCTGATCAATGTGACTATCCTCGGATCGAGAA
ATGGAGAGAGCAAATGTTTTACAAGGTGTTCAAGAGAATACAATCCCAAGCCAGTACATA
TAAGGACGATTGGGACGATGATCACCTCATCGCAGAAGCATATGAAGATTTCGTCAAATT
CCCATCTAACTATCCCAGTTCTTTAATCGAAAGAGAATATACAAGCTGAAAAACATCACT
TGAAGTCTCTGAAATGAGGGTATGTGGGTTTATTACATGCGAAATAATGCATATTGTATT
GTAAACATCACTTAAGGTCTCTGAAATGTTGGAAACTTACATGTAACAACATTACTTGAA
GTCTGAAATGTTCATGATAGGAGCGAGCTTCTTGTCTTTGAAGGCTATTCTTGTCTCAAC
AAGACAACAAGTGATTGCATCAACTTCTGTAACCTAGCAAGAAAATAACAAATAGAATTA
GTCATACAGTATTTTATAGTGACCAGACATTACCACAACGCGTCATTAGAGTAAAAGCAA
AAAACGTTAATTCAACTCACAGCTTTTACCCTCAAGGATGATGCAAGTGTTTTAAGAAAG
CATATCGAGGAAGCTTTTGTCTAATCTCCCATCATGCAACATATTAGCGACCAAGCCAAT
GGTTGAAGCATCTCCAACAAACCTGCAACTCCTCATTTCTCTGATGAGTTCAGCTGATGC
TGCTTTGTCACCATCTCTCAGATGTGCCCTGATAAGTGTATTATATGTACCACTATTTGG
AAGAGGCCCGTCTTCTTTCATTTTTTTTAGCAAGGCATAAGCTTCCTGTAATANGCGTTT
ACTACACAAGCCTGAGATCATGGTATTGTAGGTTACAACATTAGGCTTCACTCCTTTAAG
GCTGAGGCTACAAAATAGATCCCACCAATCATCCACCT
   
Sequence Name 19-K114677-0022-407-G02C-8409
GenBank Accession LN494871 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114677-0022-407-G02C-8409
TCGGATGAGCCTTTCATTGAATATGATAAATGCCCAAAAGGTTTTCAAACAGATGGTTTC
TGATGGTGTACCTCCTGATATAATGACATACAGCATTTTGTTAGATGGACTCTGTAATAA
CGGGAAGCTAGAGAAAGCATTGGAGGTTTTCGACTATATGCAAAAGAGTGAGATAAAACT
TGATATTTACATATATACTACTATGATTGAAGGGATGTGCAAGGCTGGGAAGGTGGATGA
TGGGTGGGATCTATTTTGTAGCCTCAGCCTTAAAGGAGTGAAGCCTAATGTTGTAACCTA
CAATACCATGATCTCAGGCTTGTGTAGTAAACGCCTATTACAGGAAGCTTATGCCTTGCT
AAAAAAAATGAAAGAAGACGGGCCTCTTCCAAATAGTGGTACATATAATACACTTATCAG
GGCACATCTGAGAGATGGTGACAAAGCAGCATCAGCTGAACTCATCAGAGAAATGAGGAG
TTGCAGGTTTGTTGGAGATGCTTCAACCATTGGCTTGGTCGCTAATATGTTGCATGATGG
GAGATTAGACAAAAGCTTCCTCGATATGCTTTCTTAAAACACTTGCATCATCCTTGAGGG
TAAAAGCTGTGAGTTGAATTAACGTTTTTTGCTTTTACTCTAATGACGCGTTGTGGTAAT
GTCTGGTCACTATAAAATACTGTATGACTAATTCTATTTGTTATTTTCTTGCTAGGTTAC
AGAAGTTGATGCAATCACTTGTTGTCTTGTTGAGACAAGAATAGCCTTCAAAGACAAGAA
GCTCGCTCCTATCATGAACATTTCAGACTTCAAGTAATGTTGTTACATGTAAGTTTCCAA
CATTTCAGAGACCTTAAGTGATGTTTACAATACAATATGCATTATTTCGCATGTAATAAA
CCCACATACCCTCATTTCAGAGACTTCAAGTGATGTTTTTCAG

Last Updated on 06.12.2018 16:11