Sequences from confirmation amplicon


Confirmation sequences for line 408D03 and hit At5g16500 are shown.
Line ID 408D03
Locus At5g16500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5388865
Primers used for PCR 14wm - 8474, show primer details
   
Sequence Name 78-K118585-0022-408-D03C-14wm
GenBank Accession LN513749 [GenBank]
Sequencing primer gene specific primer, 14wm: 5'-AACAACTTCAAAGAAAATCTCCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K118585-0022-408-D03C-14wm
ACTTTGGATGGTAACAACCTGGCCAGTAGATTGAAGAGTTCCTTTGTAAACCCTACCGAA
TCCACCTTCTCCTAAAAGACATTCTTGCCGGAAATTCTTGGTCGCCGTCGCTAACTCCCG
GAAATTGAATGTTTTCACCGGTGGTTGTTGCTCTGCAGGTTCTCTTTCTTCAGTCCGTTT
CGTCGTCGCAACAACTGAAACCAAACAAACCCCCCAAACAACATTTTTAGAAAATAGGTT
TTCTAATTCACAGTTCCGTGATAAGAAAGCTAACATATTTTTTTTTCTACATGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 48-K118584-0022-408-D03C-8409
GenBank Accession LN506644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118584-0022-408-D03C-8409
AGAAAAAAAATATGTTAGCTTTCTTATCACGGAACTGTGAATTAGAAAACCTATTTTCTA
AAAATGTTGTTTGGGGGGTTTGTTTGGTTTCAGTTGTTGCGACGACGAAACGGACTGAAG
AAAGAGAACCTGCAGAGCAACAACCACCGGTGAAAACATTCAATTTCCGGGAGTTAGCGA
CGGCGACCAAGAATTTCCGGCAAGAATGTCTTTTAGGAGAAGGTGGATTCGGTAGGGTTT
ACAAAGGAACTCTTCAATCTACTGGCCAGGTTTGTTACCATACCAAAGTTCAAACCTCTG
GATACAATTTTCGGAGATTTTCTTTGAAGTTGTT

Last Updated on 06.12.2018 16:11