Sequences from confirmation amplicon


Confirmation sequences for line 408D04 and hit At3g11180 are shown.
Line ID 408D04
Locus At3g11180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3506370
Primers used for PCR 07ij - 8474, show primer details
   
Sequence Name 31-K112810-0022-408-D04C-07ij
GenBank Accession LN500235 [GenBank]
Sequencing primer gene specific primer, 07ij: 5'-GTTCTCGGTCTTTAGTCGATTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112810-0022-408-D04C-07ij
ATCTTCATTCTTCCCAATCTTTTGGAATCTCTAGGTAGTTCTTTAAGCTTCCATAGCTTC
GGTATCCTTCAGTATATAGCACATAAAAGAGATTAATGTATATAGATGCACTCACTAGTA
ATTGAGCTGCATATATAAACAAGAAGGCTTACAATTATTCTATTTATCAGACATTTTATG
AAGTAGATATCAATTAACGAGGAGATATATGAGACTCAACGTGGGATTTTCCACGTGGAC
CTTGCGTTCTAATGAAGAGTCTATATTGATCAAAGGTCATGGGAGGATACAAGGGAGGCA
TCGTAGAGGTGACAAGTTGTTGCATTGGTTGGATCGGAATGTCACTCTTAGGGTTATAGA
AGAATGCTAGTGAAACCCTTTCTTTTTCCGAATTCACTATCACTCGATGTTCCACGCTCT
TGTATTTCGAATTGCTTAGTATCTGCCGGTTTTCACAAGAGAAACATATACGTTAGTATT
GAAAATTGCATCTATTGCATGACTAACATATATTGTACTTCATTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 06-K112809-0022-408-D04C-8409
GenBank Accession LN488889 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112809-0022-408-D04C-8409
TATATGTTAGTCATGCAATAGATGCAATTTTCAATACTAACGTATATGTTTCTCTTGTGA
AAACCGGCAGATACTAAGCAATTCGAAATACAAGAGCGTGGAACATCGAGTGATAGTGAA
TTCGGAAAAAGAAAGGGTTTCACTAGCATTCTTCTATAACCCTAAGAGTGACATTCCGAT
CCAACCAATGCAACAACTTGTCACCTCTACGATGCCTCCCTTGTATCCTCCCATGACCTT
TGATCAATATAGACTCTTCATTAGAACGCAAGGTCCACGTGGAAAATCCCACGTTGAGTC
TCATATATCTCCTCGTTAATTGATATCTACTTCATAAAATGTCTGATAAATAGAATAATT
GTAAGCCTTCTTGTTTATATATGCAGCTCAATTACTAGTGAGTGCATCTATATACATTAA
TCTCTTTTATGTGCTATATACTGAAGGATACCGAAGCTATGGAAGCTTAAAGAACTACCT
AGAGATTCCAAAAGATTGGTGAAGAATGAAGATATATATACATATATATTGAGATCAAAG
TGAATCGACTAAGACCCGAGAA

Last Updated on 06.12.2018 16:11