Sequences from confirmation amplicon


Confirmation sequences for line 408F08 and hit At1g07530 are shown.
Line ID 408F08
Locus At1g07530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2315176
Primers used for PCR 10ij - 8474, show primer details
   
Sequence Name 32-K112810-0022-408-F08C-10ij
GenBank Accession LN500652 [GenBank]
Sequencing primer gene specific primer, 10ij: 5'-GCTAGCACCACTACCACTACTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112810-0022-408-F08C-10ij
ACCTTCTTGGTTCATACACCTATGCCGAGTAATTTTGTTTTCCAGTCTACTTCTAGGTCC
AACAGTGTCACCGGTGGTGGTGGTGGTGGTAATAGTGCGGTTTACGGTTCAGGTTTTGGC
GATGATTTGGTTTCGAATATGTTTAAAGATGATGAATTGGCTATGCAGTTCAAGAAAGGG
GTTGAGGAAGCTAGTAAGTTCCTTCCTAAGTCTTCTCAGCTCTTTATTGATGTGGATAGT
TACATCCCTATGAATTCTGGTTCCAAGGAAAATGGTTCTGAGGTTTTTGTTAAGACGGAG
AAGAAAGATGAGACAGAGCATCATCATCATCATAGCTATGCTCCTCCTCCCAACAGATTA
ACTGGTAAGAAAAGCCATTGGCGCGACGAAGATGAAGATTTCGTTGAAGAAAGAAGTAAC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCC
GCAGCGTTATA
   
Sequence Name 07-K112809-0022-408-F08C-8409
GenBank Accession LN489349 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112809-0022-408-F08C-8409
CATTTACATTGTTACTTCTTTCTTCACGAAATCTTCATCTTCGTCGCGCCAATGGCTTTT
CTTACCAGTTAATCTGTTGGGAGGAGGAGCATAGCTATGATGATGATGATGCTCTGTCTC
ATCTTTCTTCTCCGTCTTAACAAAAACCTCAGAACCATTTTCCTTGGAACCAGAATTCAT
AGGGATGTAACTATCCACATCAATAAAGAGCTGAGAAGACTTAGGAAGGAACTTACTAGC
TTCCTCAACCCCTTTCTTGAACTGCATAGCCAATTCATCATCTTTAAACATATTCGAAAC
CAAATCATCGCCAAAACCTGAACCGTAAACCGCACTATTACCACCACCACCACCACCGGT
GACACTGTTGGACCTAGAAGTAGACTGGAAAACAAAATTACTCGGCATAGGTGTATGTAA
CCAAGAAGGTCTATTCTCCAAACCATCAACACTCCAGTGAGAATCAGAGGAAGTAGTGGT
AGTGGTGCTAG

Last Updated on 06.12.2018 16:11