Genotyping and primer details


Genotyping and primer details for 408F08 - At1g07530
Line ID 408F08
Locus At1g07530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2315176 of line 408F08 Chr1:2315176
   
Insertion allele amplicon 8474 - 10ij
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ij: 5'-GCTAGCACCACTACCACTACTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2315660
   
wt amplicon (over InsPos) TA26 - 10ij
wt amplicon start Chr1:2314908
wt amplicon end Chr1:2315660
wt amplicon size 752 bp
Locus specific primer 10ij: 5'-GCTAGCACCACTACCACTACTTCC-3'
2nd locus specific primer TA26: 5'-TCTACGATCATCCACTGATACAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11