Sequences from confirmation amplicon


Confirmation sequences for line 408H06 and hit At5g50570 are shown.
Line ID 408H06
Locus At5g50570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20583349
Primers used for PCR 17oi - 8474, show primer details
   
Sequence Name 49-K114716-0022-408-H06C-17oi
GenBank Accession LN506930 [GenBank]
Sequencing primer gene specific primer, 17oi: 5'-CATGTGAACCACCAGAAAACTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K114716-0022-408-H06C-17oi
CTTCTTATCAACAAGGAACGGTCTATTGGATTCAAGAAACTAACTAACTTTTCTTAAAAC
AGAAACCTTGAAAACCCGTAAAGAAGTTGGCAGGACGACCGATATGTTCAGGCTGCGGCT
TCCGTCGTCTTCGATTATGTCCATCAAGACGTTTCCTACAACTTCTCTTCCCTTCATCAA
ACTCCTCCAAAGCATGAAACCTAATTAGACACAAATGTTAACATCTTCACCAACCCAACA
AGAAGCTTAAACCATTAACACACAATCTTCATGTCCGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTATA
   
Sequence Name 01-K114715-0022-408-H06C-8409
GenBank Accession LN486682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114715-0022-408-H06C-8409
TTGAATATATCCGGCACTGAAGATTGTGTGTTAATGGTTTAAGCTTCTTGTTGGGTTGGT
GAAGATGTTAACCTTTGTGTCTAATTAGGTTTCATGCCTTGGAGGAGTTTGACGAACGGA
ACAGAAGTTGTATGAAACGTCTTGTTCGACCTAATCGAAGACGACGGACTC

Last Updated on 06.12.2018 16:11