Sequences from confirmation amplicon


Confirmation sequences for line 409E02 and hit At4g24140 are shown.
Line ID 409E02
Locus At4g24140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12530355
Primers used for PCR NT48 - 8474, show primer details
   
Sequence Name 35-K112551-0022-409-E02C-NT48
GenBank Accession LN501886 [GenBank]
Sequencing primer gene specific primer, NT48: 5'-TCTCTAGGACAAATGGGGACTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112551-0022-409-E02C-NT48
GCTAATTTTGAATTCCACCTAAGTACTTATGACTATGTGTAATTGGAGCAATTTAATTGA
TCTTACTTTTGAATTGTCTACGTTGGTTGATCGGCCTATTTCAGTGACATGTCCGTATAG
ATTTTACGATTCTATTCTATAGTTTCTACTTTATAGGTATTACAAGTAAAATACATGGTT
TAGAAATAGTGCTCTCGTCCTACCAAAACAACCAACCCTTCGAGATTAAATAAAATATAG
AAAATGGAATAAAAATTCCTCCGATTAGTGCTACCAGATATGAGTTCAACTCCAAAATCA
ATAGTAGCTATNAATTTCGTAATTAATATTTGAAAAATTAAATTCAATCAAGTTTAAATA
ATCATCATCAGGAATAAGTAAATTAGCTACGTTGAGATGCAAACTCTATGACTCTTTATT
GTCATATCATCTAGTACGAAACTACATTTTGTCATATCATCTAGTTTACTAGCTAATAAT
AAATATATTTTCTCTATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 11-K112550-0022-409-E02C-8409
GenBank Accession LN491183 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112550-0022-409-E02C-8409
TGAATTATCCTGGATAGAGAAATATATTTATTATTAGCTAGTAAACTAGATGATATGACA
AAATGTAGTTTCGTACTAGATGATATGACAATAAAGAGTCATAGAGTTTGCATCTCAACG
TAGCTAATTTACTTATTCCTGATGATGATTATTTAAACTTGATTGAATTTAATTTTTCAA
ATATTAATTACGAAATTAATAGCTACTATTGATTTTGGAGTTGAACTCATATCTGGTAGC
ACTAATCGGAGGAATTTTTATTCCATTTTCTATATTTTATTTAATCTCGAAGGGTTGGTT
GTTTTGGTAGGACGAGAGCACTATTTCTAAACCATGTATTTTACTTGTAATACCTATAAA
GTAGAAACTATAGAATAGAATCGTAAAATCTATACGGACATGTCACTGAAATAGGCCGAT
CAACCAACGTAGACAATTCAAAAGTAAGATCAATTAAATTGCTCCAATTACACATAGTCA
TAAGTACTTAGGTTGGAATTTCAAAATTTAGCATAACACTAGAGTCAGGTTTGTTTGGTT
GCCTAAGTCCCCATTGTCCCCTAGAGA

Last Updated on 06.12.2018 16:11