Sequences from confirmation amplicon


Confirmation sequences for line 409E04 and hit At2g29065 are shown.
Line ID 409E04
Locus At2g29065 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12486781
Primers used for PCR 37sj - 8474, show primer details
   
Sequence Name 57-K116453-0022-409-E04C-37sj
GenBank Accession LN509518 [GenBank]
Sequencing primer gene specific primer, 37sj: 5'-AGAACCTTCAAGATGAAACAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K116453-0022-409-E04C-37sj
GCTATAAGAACATGAACCCGGACGTTTTCATCCACGCGATTGTCAACGGTTCATTCAACG
CACCCTTCTTTATCTCGCGGTTTAAAGAAGCGGTTTACCATTACTCCGCTCTCTTCGACA
TGTTTGATTCGACGTTGCCTCGGGATAACAAAGAGAGGATTAGGTTCGAGAGGGAGTTTT
ACGGGAGAGAGGCTATGAACGTGATAGCGTGCGAGGAAGCTGATCGAGTGGAGAGGCCTG
AGACTTACAGGCAATGGCAGGTTAGAATGGTTAGAGCCGGGTTTAAATTCAATTGATTCA
TGTAGATTTGCTTCATTTACAGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 24-K116452-0022-409-E04C-8409
GenBank Accession LN497142 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116452-0022-409-E04C-8409
ATTTACATTGAATACTGTAAATGAAGCAAATCTACATGAATCAATTGAATTTAAACCCGG
CTCTAACCATTCTAACCTGCCATTGCCTGTAAGTCTCAGGCCTCTCCACTCGATCAGCTT
CCTCGCACGCTATCACGTTCATAGCCTCTCTCCCGTAAAACTCCCTCTCGAACCTAATCC
TCTCTTTGTTATCCCGAGGCAACGTCGAATCAAACATGTCGAAGAGAGCGGAGTAATGGT
AAACCGCTTCTTTAAACCGCGAGATAAAGAAGGGTGCGTTGAATGAACCGTTGACAATCG
CGTGGATGAAAACGTCCGGGTTCATGTTTCTTATTAGCTTCAAGACAGCATCTCTCGGGC
AATTCTCTTCGCTTCCTGTTTCACTGGAAAGGTTCT

Last Updated on 06.12.2018 16:11