Sequences from confirmation amplicon


Confirmation sequences for line 411G05 and hit At1g74380 are shown.
Line ID 411G05
Locus At1g74380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27960107
Primers used for PCR MV02 - 8474, show primer details
   
Sequence Name 13-K026965-022-411-G05C-8409
GenBank Accession LN491882 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K026965-022-411-G05C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACATGAAGCC
ATTTTCAATGATGTTCTGGTTAACTGCATCGGCGCTTGAGCTTCCTAGGTTACCAACACC
GATAGTGCCACGTAAGACGAGAATTGTGACGAAACCACAGAGAATTGTGATCTTGATGTT
GTTAAACGTCTTCTGCATCTGTCTACCTCGAGGTAACAAACCACCACGACCACCTCTTCC
TCCACCGTTGGTTAACGTCGTCGTCGGAAGTCCTCCGCCGCTTCCGGATGGTCTTTTATG
CGCCGGCGAACCATCTTGACCCATTTTCTTCTTCAACGTCTCTCTCTATAGATTTCACTT
ACAGGTTTGCGACCATCAACTTTGTTTTCCTCAGATCAGTCTGAGTATGTTTCTGTCTCT
CCGTACTTACTTATTCACGGATCTGAGAAAAAGTTCAGATCTTTAGCATGATTCCGATCG
GGGAAAGAGAAAGAGAAAGATTTGTCGATCTG
   
Sequence Name 14-K026965-022-411-G05C-MV02
GenBank Accession LN492351 [GenBank]
Sequencing primer gene specific primer, MV02: 5'-TTAGGGTTCTTCAGATCGACAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K026965-022-411-G05C-MV02
TCTTTTCTTTCCCCGATCGGAATCATGCTAAAGATCTGAACTTTTTCTCAGATCCGTGAA
TAAGTAAGTACGGAGAGACAGAAACATACTCAGACTGATCTGAGGAAAACAAAGTTGATG
GTCGCAAACCTGTAAGTGAAATCTATAGAGAGAGACGTTGAAGAAGAAAATGGGTCAAGA
TGGTTCGCCGGCGCATAAAAGACCATCCGGAAGCGGCGGAGGACTTCCGACGACGACGTT
AACCAACGGTGGAGGAAGAGGTGGTCGTGGTGGTTTGTTACCTCGAGGTAGACAGATGCA
GAAGACGTTTAACAACATCAAGATCACAATTCTCTGTGGTTTCGTCACAATTCTCGTCTT
ACGTGGCACTATCGGTGTTGGTAACCTAGGAAGCTCAAGCGCCGATGCAGTTAACCAGAA
CATCATTGAAAATGGCTTCATGTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11