Sequences from confirmation amplicon


Confirmation sequences for line 412D08 and hit At4g34440 are shown.
Line ID 412D08
Locus At4g34440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16466034
Primers used for PCR RK81 - 8474, show primer details
   
Sequence Name 27-K021337-022-412-D08C-8409
GenBank Accession LN498190 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K021337-022-412-D08C-8409
TTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCCCTGAAACCTCAAATGGG
ACACCACCGTCAAACGGAACATCGCCGTCTAATGAGTCATCGCCGCCAACACCACCTTCT
TCACCACCACCATCATCAATATCTGCTCCTCCGCCAGATATCTCCGCTTCTTTTTCACCG
CCGCCTGCACCACCAACGCAAGAAACGTCACCTCCTACATCTCCGTCCTCATCGCCGCCT
GTTGTAGCTAATCCGTCACCGCAGACTCCAGAGAATCCTTCTCCACCTGCACCTGAAGGC
TCAACTCCTGTAACGCCACCTGCACCACCACAAACACCGTCGAACCAATCACCGGAAAGA
CCAACTCCTCCTTCTCCTGGTGCCAATGATGACCGAAACAGAACCAATGGCGGAAACAAC
AACAGAGACGGCTCCACACCATCACCACCGTCGTCAGGGAACAGAACTTCCGGTGACGGT
GGCTCACCTTCACCACCTCGGTCGATAAGCCCTCCTCAGAATAGTGGAGATTCAGACTCA
TCATCGGGTAATCATCCACAAGCCAACATTGGATTGATTATTGGAGTCCTTGTAGGAGCA
GGGCTTTTGCTTCTACTTGCAGTGTGTATTTGCATCTGTTGC
   
Sequence Name 28-K021337-022-412-D08C-RK81
GenBank Accession LN498660 [GenBank]
Sequencing primer gene specific primer, RK81: 5'-TGATCGTTCTGAAACCTAATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K021337-022-412-D08C-RK81
TTAGGCTACAAATTAAATTCCAGTTATACTAAACTGTAATTACCTGAGGGTGCTCCTCCA
TAAGGATTGTTATTGTAGTAGTGCATGTGGTTGACCTGAGGAGATTTCTTCTTCTTCTTC
CTGTTGCAACAGATGCAAATACACACTGCAAGTAGAAGCAAAAGCCCTGCTCCTACAAGG
ACTCCAATAATCAATCCAATGTTGGCTTGTGGATGATTACCCGATGATGAGTCTGAATCT
CCACTATTCTGAGGAGGGCTTATCGACCGAGGTGGTGAAGGTGAGCCACCGTCACCGGAA
GTTCTGTTCCCTGACGACGGTGGTGATGGTGTGGAGCCGTCTCTGTTGTTGTTTCCGCCA
TTGGTTCTGTTTCGGTCATCATTGGCACCAGGAGAAGGAGGAGTTGGTCTTTCCGGTGAT
TGGTTCGACGGTGTTTGTGGTGGTGCAGGTGGCGTTACAGGAGTTGAGCCTTCAGGTGCA
GGTGGAGAAGGATTCTCTGGAGTCTGCGGTGACGGATTAGCTACAACAGGCGGCGATGAG
GACGGAGATGTAGGAGGTGACGTTTCTTGCGTTGGTGGTGCAGGCGGCGGTGAAAAAGAA
GCGGAGATATCTGGCG

Last Updated on 06.12.2018 16:11