Sequences from confirmation amplicon


Confirmation sequences for line 412E06 and hit At4g00430 are shown.
Line ID 412E06
Locus At4g00430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:186654
Primers used for PCR CC12 - 8474, show primer details
   
Sequence Name 27-K106138-0022-412-E06C-8409
GenBank Accession LN498395 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K106138-0022-412-E06C-8409
GCCATTACGTGTAGACGAGAACGAATGTTCCGATGATTTCAGCACCAAGGCCAGAACCTT
TGGTGTAGCCATGAGCAACGGTGTTAGCACCACCACCGAGAGTCTGGTACGGCGTTGGCT
GGAAACCTTTGACGACTCCGGCACCACAGATGGCTCCAAGACATTGCATAATCATGTAGA
ACACTGCTCTGGTCAGAGATAACTTCCGAGCCAAGAACAGACCGAATGTTACAGCAGGGT
TGATGTGTCCACCTAAAAACACACAAACACAAACACATGTTACACAAAGATGTGAGCTTT
ATCTGAGAAATCAACAGTAAAATTGGTGTAACATACAGATCTAGATAAGCCCAAGCATCA
AAAGAACCCAAAGTTTCCTGCTTTATAACTGTTTTTGGTTCCTTTACATTTTCTAGGTCA
AACAGAGCATGAACAAAAACAGAGGACTCACATAATGGTTTTCAGAAACAAAACAGAGCA
AATAAAGCAGAACCAAAACAGAGAACCCACCAAAACAGAGATTACAGAAAAATGGGAAAA
TCGAGAGAAGGTTACCTGAAATTCCAGCAGTACAGTAGACAAGAGCAAAGATCATGCCAC
CAAAAGCCCAAGCGATACCTTGGATTCCAACAGAAGCACACATGTTTGGTGCTCTCTTAA
CTCCCATTACAGTCAAAACAGTGATGTAGAGAAACAAGAAAGTAGCGATAAACTCAGCTA
TTCCGGCTCTGTAGAAAGACCATGAGCTGAGCTCGCCGGGCTCGAACAGTGGCGCAGGAG
GTGGCTCTNTGTAGTCCTTGTCGGTGGACTGAGCCGATGTACCGATGGGTTGCCTCTCCN
GGAACTTGTTAGCTCCC
   
Sequence Name 27-K106137-0022-412-E06C-CC12
GenBank Accession LN498394 [GenBank]
Sequencing primer gene specific primer, CC12: 5'-AAAGAAGAAGATGTACGAGTGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K106137-0022-412-E06C-CC12
CATCAGTACTCGGCTCAGTCCACCGACAGGACTACAAAGAGCCACCTCCTGCGCCACTGT
TCGAGCCCGGCGAGCTCAGCTCATGGTCTTTCTACAGAGCCGGAATAGCTGAGTTTATCG
CTACTTTCTTGTTTCTCTACATCACTGTTTTGACTGTAATGGGAGTTAAGAGAGCACCAA
ACATGTGTGCTTCTGTTGGAATCCAAGGTATCGCTTGGGCTTTTGGTGGCATGATCTTTG
CTCTTGTCTACTGTACTGCTGGAATTTCAGGTAACCTTCTCTCGATTTTCCCATTTTTCT
GTAATCTCTGTTTTGGTGGGTTCTCTGTTTTGGTTCTGCTTTATTTGCTCTGTTTTGTTT
CTGAAAACCATTATGTGAGTCCTCTGTTTTTGTTCATGCTCTGTTTGACCTAGAAAATGT
AAAGGAACCAAAAACAGTTATAAAGCAGGAAACTTTGGGTTCTTTTGATGCTTGGGCTTA
TCTAGATCTGTATGTTACACCAATTTTACTGTTGATTTCTCAGATAAAGCTCACATCTTT
GTGTAACATGTGTTTGTGTTTGTGTGTTTTTAGGTGGACACATCAACCCTGCTGTAACAT
TCGGTCTGTTCTTGGCTCGGAAGTTATCTCTGACCAGAGCAGTGTTCTACATGATTATGC
AATGTCTTGGAGCCATCTGTGGTGCCGGAGTCGTCAAAGGTTTCCAGCCAACGCCGTACC
AGACTCTCGGTGGTGGTGCTAACACCGTTGCTCATGGCTACACCAAAGGTTCTGGCCTTG
GTGCTGAAATCATCGGAACATTCGTTCTCGTCTACACTGTAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTACC
CATTTTTTTTCATTCAAAAT

Last Updated on 06.12.2018 16:11