Sequences from confirmation amplicon


Confirmation sequences for line 413A11 and hit At5g15510 are shown.
Line ID 413A11
Locus At5g15510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5035595
Primers used for PCR 14wu - 8474, show primer details
   
Sequence Name 39-K118761-0022-413-A11C-14wu
GenBank Accession LN503525 [GenBank]
Sequencing primer gene specific primer, 14wu: 5'-CACTATCAGCTATTTTCCTTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118761-0022-413-A11C-14wu
GTATACTCTAAATGATTTGACAGAATGAAATGGTTACTGAAGCCAAATCATGTCAGCGGC
TATGTTTTAAGGTATCTTGAACTTCAAGCCCAACAAAATGTAGATTTAATCATTGCATTA
GCAAGGTTACCTGGTAATCAAATTCTGCGCGTTCAACTGCCCGGACATCTGAGTGGAGCG
TCAAGTCGACTGGTCTTGTAATGTCTTTCCAATGAGGCTTCACTAGGCACTGAAACAGAA
AACAACATTAGAACTTTTCATAGTCAATGGCTACATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 14-K118760-0022-413-A11C-8409
GenBank Accession LN492731 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118760-0022-413-A11C-8409
ATTTACAATTGAATATGTAGCCATTGACTATGAAAAGTTCTAATGTTGTTTTCTGTTTCA
GTGCCTAGTGAAGCCTCATTGGAAAGACATTACAAGACCAGTCGACTTGACGCTCCACTC
AGATGTCCGGGCAGTTGAACGCGCAGAATTTGATTACCAGGTAACCTTGCTAATGCAATG
ATTAAATCTACATTTTGTTGGGCTTGAAGTTCAAGATACCTTAAAACATAGCCGCTGACA
TGATTTGGCTTCAGTAACCATTTCATTCTGTCAAATCATTTAGAGTATACTTGTTTTCTT
ATCAGCTGGTTGTGTTAAGACTTGAGACGGAAAGGAAAATAGCTGATAGTG

Last Updated on 06.12.2018 16:11