Sequences from confirmation amplicon


Confirmation sequences for line 413B06 and hit At3g26490 are shown.
Line ID 413B06
Locus At3g26490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9704788
Primers used for PCR 18us - 8474, show primer details
   
Sequence Name 46-K117387-0022-413-B06C-18us
GenBank Accession LN505989 [GenBank]
Sequencing primer gene specific primer, 18us: 5'-CCAAGTCAAATCCACAAAATACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117387-0022-413-B06C-18us
CATTTCATGTTTTGATTCTGATTTTTTTGTGTTTCTTGGCAATGCAATTTACAGTTTCCG
ATGTTATCCAAATGCTTACGTTTAAAGAACTTAGTCTCTTCACAAGAAACAGAAACCTCT
CAAGAACAGCAAGTCATTCAACTTGTTGATTTTCCCGGTGAAACAGAAGCTTTTGAGCTT
TGTGCTAAGTTCTGTTACGGCATCACAATCACTCTCTGTGCTCACAATGTAGTCGCGGTA
CGTTGCGCAGCAGAGTATCTAGGGATGACGGAAGAAGTGGAGCTCGGAGAGACGGAGAAT
CTTGTTCAGAGACTTGAACTTTTCTTGACTACTTGTGTGTTCAAGAGTTGGAGAGATTCT
TATGTTACTCTTCAGACTACAAAGGTTAATCATCACACCGGGAAATGGCTTCATGTCCGG
AGAACAATGGCTTCAATTGTAATTACAATGATACAATGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 19-K117386-0022-413-B06C-8409
GenBank Accession LN494935 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117386-0022-413-B06C-8409
ATATCATTGTATCATTGTAATTACAATTGAAGCCATTGTTCTCCGGACATGAAGCCATTT
CCCGGTGTGATGATTAACCTTTGTAGTCTGAAGAGTAACATAAGAATCTCTCCAACTCTT
GAACACACAAGTAGTCAAGAAAAGTTCAAGTCTCTGAACAAGATTCTCCGTCTCTCCGAG
CTCCACTTCTTCCGTCATCCCTAGATACTCTGCTGCGCAACGTACCGCGACTACATTGTG
AGCACAGAGAGTGATTGTGATGCCGTAACAGAACTTAGCACAAAGCTCAAAAGCTTCTGT
TTCACCGGGAAAATCAACAAGTTGAATGACTTGCTGTTCTTGAGAGGTTTCTGTTTCTTG
TGAAGAGACTAAGTTCTTTAAACGTAAGCATTTGGATAACATCGGAAACTGTAAATTGCA
TTGCCAAGAAACACAAAAAAATCAGAATCAAAACATGAAATGTTATAAGAAACAACACAA
AACAAGAAAAAAAAACCTTGTGAAGAAGGTATTTTGTGATTTGGACTTGG

Last Updated on 06.12.2018 16:11