Sequences from confirmation amplicon


Confirmation sequences for line 413F08 and hit At4g08310 are shown.
Line ID 413F08
Locus At4g08310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5249221
Primers used for PCR CE05 - 8474, show primer details
   
Sequence Name 08-K106018-0022-413-F08C-CE05
GenBank Accession LN489735 [GenBank]
Sequencing primer gene specific primer, CE05: 5'-ATTATTCGATCAAAGACGGAATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106018-0022-413-F08C-CE05
GCAAGAGCAACTATTGGGGAAAAACTGACAAATCATATCCCTTCTAAATTTCAGATGATA
ACTAATTCAGTGTTATAATGCAAGCCTTAAAATTCATATCCTAATGTCATAAATATGATT
AGCACATTAAAAAGGTACAAAATATCACCACTCTACAACAGTACATAGTGAAACTAGGCT
ACATTACAAACAACCAACGATCTTACAATTATTCGCTTTCTTCTTCACCACCATCCTCTG
CAACCAAACCAAAATAACCATCAGTGTTTAAAAACACCTTCTGCTGAAATTTGATGTAAA
CAATTTACAGAAGACGAGTTAGATTCCTACCAGTTTTTAGTTCTCCTTCGTTTTGACTTC
CCTCTCCGCCATCTTCACTACCTCCTCCTCCTCTTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 08-K106019-0022-413-F08C-8409
GenBank Accession LN489736 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106019-0022-413-F08C-8409
CCATTTACATTGAAGAGGAGGAGGAGGTAGTGAAGATGGCGGAGAGCGATGTCAAAACGA
AGGAGAACTAAAAACTGGTAGGAATCTAACTCGTCTTCTGTAAATTGTTTACATCAAATT
TCAGCAGAAGGTGTTTTTAAACACTGATGGTTATTTTGGTTTGGTTGCAGAGGATGGTGG
TGAAGAAGAAAGCGAATAATTGTAAGATCGTTGGTTGTTTGTAATGTAGCCTAGTTTCAC
TATGTACTGTTGTAGAGTGGTGATATTTTGTACCTTTTTAATGTGCTAATCATATTTATG
ACATTAGGATATGAATTTTAAGGCTTGCATTATAACACTGAATTAGTTTTCATCTGAAAT
TTAGAAGGGATATGATTTTGTCAGTTTTTTCCCCAATATGTTTGCTTCATTTGGCCCTAA
CTCATTTTCCAATTCCGTCTTTGATCGAATAA
Primers used for PCR CE06 - 8474, show primer details
   
Sequence Name 53-K118893-0022-413-F08C-CE06
GenBank Accession LN508313 [GenBank]
Sequencing primer gene specific primer, CE06: 5'-TCTTTCTTGGCAGTATTTCTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118893-0022-413-F08C-CE06
CGAGGAATTCTGATTAAAGAACTTAAGGAGTTACTCGCCAAAGAAGGGTTGTCTGCAAAT
CCTTCAGAGAAAGGTAATGCTTTACATTTAAGAAGTCTCCTCTACCTGCAGGAAAGCGTA
GACGCCTTCAAGATATTCTAGAGCGAAAAATATGCGGATGATAAAATTGTTGATGGTTTT
ACCTGGTGACAGAGATCAAGGAAGTCAAGAAAAGAAAAGAAAGAACAAAGGAGCTTGAGG
GTATTGATACAAGCAACATTGTGTCGAGTTCAAGGAGAAGATCCAGCGCAAGTTTTGTAC
CTCCTCCAAAGCCGATAAAAGCAGAAGAAAGCGAGAGCGATGATTCAGAAGATTCTGAGA
ATGAAGAGGATGAGGATGAAGAAGTAGTAGTAGAAGAAGAAGAGGAGGAAGAAGACGAGG
GAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATT
   
Sequence Name 13-K118892-0022-413-F08C-8409
GenBank Accession LN492272 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118892-0022-413-F08C-8409
TTACATTGAATATATTCCCTCGTCTTCTTCCTCCTCTTCTTCTTCTACTACTACTTCTTC
ATCCTCATCCTCTTCATTCTCAGAATCTTCTGAATCATCGCTCTCGCTTTCTTCTGCTTT
TATCGGCTTTGGAGGAGGTACAAAACTTGCGCTGGATCTTCTCCTTGAACTCGACACAAT
GTTGCTTGTATCAATACCCTCAAGCTCCTTTGTTCTTTCTTTTCTTTTCTTGACTTCCTT
GATCTCTGTCACCAGGTAAAACCATCAACAATTTTATCATCCGCATATTTTTCGCTCTAG
AATATCTTGAAGGCGTCTACGCTTTCCTGCAGGTAGAGGAGACTTCTTAAATGTAAAGCA
TTACCTTTCTCTGAAGGATTTGCAGACAACCCTTCTTTGGCGAGTAACTCCTTAAGTTCT
TTAATCAGAATTTCCTCGCGTTTCTCCTCAGGAGCCTGCTTGGCTTTCCTGTAAACCGAT
GGAGAAATACTGCCAAGAAAGA

Last Updated on 06.12.2018 16:11