Sequences from confirmation amplicon


Confirmation sequences for line 414B11 and hit F9E21 are shown.
Line ID 414B11
Locus F9E21 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:3149712
Primers used for PCR 19hm - 8474, show primer details
   
Sequence Name 39-K112562-0022-414-B11C-19hm
GenBank Accession LN503404 [GenBank]
Sequencing primer gene specific primer, 19hm: 5'-AGATGACTTTGTCAGCCATTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K112562-0022-414-B11C-19hm
GAACTGACAAGTTGATAAGCTCCTTCTGTTTTGTGCTCCTTGTTTGCCTAGGCATACACC
TAAACACACAGAAAAAGAAGAAAACAAGAAACGTGTGAGTAATAAAATAGAAGTAAAAAC
TAGATGAAATCTCTAAACAAAACTCAATGATGATTCCCAAAAACTTCGGCAACGGCGCCA
AATTTGATATGGTTCAAATTACCTAGAACTACTCTCTTAAATAAGAGGTCAATTGCAGTA
TTTTAGGATCGAATTCACGGAGTTCTTTTCTTCAAACAGTGAGTTAAATGTGGAGATTAA
GCTAGCAGGGTACGAGTGAAAATAAAAGAGAACAAAGTAAAAAGACAGCAGATTGATTGT
GTTGTGAACGATTTAATAAAGAGCTAGGAACAAAGTATTCTCAAGAAACTATTGGTTAGT
AGATCTAATGAAAGCTAGGTTTTTATCGAACCATTCTTAAACTCAAACTCTAATTATGGA
ATAATCGGTGGGCGTGCCGCGAAACTCCCTATATCTATAGCTAATAATAACCGGAAAAGC
CGAGAAACTATCCACCTAAATGTGCATTCTTACCGTACATAGGTGTGGCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
G
   
Sequence Name 15-K112561-0022-414-B11C-8409
GenBank Accession LN493040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K112561-0022-414-B11C-8409
TTGAATATATCCTGCCACACCTATGTACGGTAAGAATGCACATTTAGGTGGATAGTTTCT
CGGCTTTTCCGGTTATTATTAGCTATAGATATAGGGAGTTTCGCGGCACGCCCACCGATT
ATTCCATAATTAGAGTTTGAGTTTAAGAATGGTTCGATAAAAACCTAGCTTTCATTAGAT
CTACTAACCAATAGTTTCTTGAGAATACTTTGTTCCTAGCTCTTTATTAAATCGTTCACA
ACACAATCAATCTGCTGTCTTTTTACTTTGTTCTCTTTTATTTTCACTCGTACCCTGCTA
GCTTAATCTCCACATTTAACTCACTGTTTGAAGAAAAGAACTCCGTGAATTCGATCCTAA
AATACTGCAATTGACCTCTTATTTAAGAGAGTAGTTCTAGGTAATTTGAACCATATCAAA
TTTGGCGCCGTTGCCGAAGTTTTTGGGAATCATCATTGAGTTTTGTTTAGAGATTTCATC
TAGTTTTTACTTCTATTTTATTACTCACACGTTTCTTGTTTTCTTCTTTTTCTGTGTGTT
TAGGTGTATGCCTAGGCAAACAAGGAGCACAAAACAGAAGGAGCTTATCAACTTGTCAGT
TCTGGACTTGGGAAAACTTGAACGCACCAACCGAAAAACTCAGAAATTAACAATGGCTGA
C

Last Updated on 06.12.2018 16:11