Sequences from confirmation amplicon


Confirmation sequences for line 414C02 and hit At1g07810 are shown.
Line ID 414C02
Locus At1g07810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2417970
Primers used for PCR RM12 - 8474, show primer details
   
Sequence Name 07-K027360-022-414-C02C-8409
GenBank Accession LN489121 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K027360-022-414-C02C-8409
GGGTCGGATGGATGCAAATCTTCAGATGATTGCAAAAATTGCTGCCATTTGCAACGATGC
TAATGTCGAGCAATCTGATCAGCAATTTGTGTCTAGGGGCATGCCTACTGAGGCAGCTTT
GAAGGTAAAGCGGTCTACTACACTAGCTTGCACCTTTCTCTTCTTAACTAAATTGACTAA
GAAAACTCTATTCGTTGTCCTTAGTTGATGTTTCTTTCTATTGGTGTGTGTGTTTATTTG
AACAGGTTTTGGTTGAGAAAATGGGATTTCCCGAAGGATTGAATGAAGCTTCGTCCGATG
GCGATGTCTTACGTATGTATTCTGAACTATATCTGTTACTTATGTTTATGCAGTTTTACA
GATGCCTAACCTCCTGCACTTTTCCTTTTTTAACATTATTGAAGGTTGTTGTCGGCTATG
GAGTGAACTAGAACAACGGATTGCCACCCTTGAGTTTGACCGTGACCGGAAATCAATGGG
AGTTATGGTGGATTCCAGCTCAGGAAACAAA
   
Sequence Name 08-K027360-022-414-C02C-RM12
GenBank Accession LN489583 [GenBank]
Sequencing primer gene specific primer, RM12: 5'-ACCTTGACCAGTAGCAGTTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K027360-022-414-C02C-RM12
TCAAACTCAAGGGTGGCAATCCGTTGTTCTAGTTCACTCCATAGCCGACAACAACCTTCA
ATAATGTTAAAAAAGGAAAAGTGCAGGAGGTTAGGCATCTGTAAAACTGCATAAACATAA
GTAACAGATATAGTTCAGAATACATACGTAAGACATCGCCATCGGACGAAGCTTCATTCA
ATCCTTCGGGAAATCCCATTTTCTCAACCAAAACCTGTTCAAATAAACACACACACCAAT
AGAAAGAAACATCAACTAAGGACAACGAATAGAGTTTTCTTAGTCAATTTAGTTAAGAAG
AGAAAGGTGCAAGCTAGTGTAGTAGACCGCTTTACCTTCAAAGCTGCCTCAGTAGGCATG
CCCCTAGACACAAATTGCTGATCAGATTGCTCGACATTAGCATCGTTGCAAATGGCAGCA
ATTTTTGCAATCATCTGAAGATTTGCATCCATCCGACCCATTGGCCAATCTTCATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11