Sequences from confirmation amplicon


Confirmation sequences for line 414D11 and hit At2g28990 are shown.
Line ID 414D11
Locus At2g28990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12455314
Primers used for PCR EQ58 - 8474, show primer details
   
Sequence Name 23-K103075-0022-414-D11C-EQ58
GenBank Accession LN496513 [GenBank]
Sequencing primer gene specific primer, EQ58: 5'-AGATCATCTGGCGATAAACCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K103075-0022-414-D11C-EQ58
ATACACTATTCAACACCAGATTGATTATTTCCACGATCATTATTTAATTTTCTACCCGGA
GTTGACTTTTGTATATTTCATTCCAACTTATACATGAGGTATCTCTCAGTTCCATATCTT
TTGCAGTTTTCCCTAAGCAAGTCTTTGTTTTTTCTTGAGTACTAGAGAAACCGGATAGTG
ATCGAGAGAGAACATGAAGATTCACCTTTTGTTGGCCATGATTGGAACCTTTGTCGTTAT
AATTGGAGCCCAGGATCAAGAAGGTAATGATATATACCATCTCTTTCTTGTTTATAAATC
AATCCTTTGACTTGTGATATGTTATATAAGAATGTATCAATATCACTTTTCCCATTTATT
GCTTTTATATTTGAACAGGTTTCATCAGTTTAGATTGTGGATTACCTAGTGACGAATCTC
CTTACGATGATTCGTTTAACGGATTTAACTACATGAAAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 24-K103075-0022-414-D11C-8409
GenBank Accession LN496950 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K103075-0022-414-D11C-8409
TCGGACTGACCATTTTTCTGTAGTTAATCCGTTAAACGAATCATCGTAAGGAGATTCGTC
ACTAGGTAATCCACAATCTAAACTGATGAAACCTGTTCAAATATAAAAGCAATAAATGGG
AAAAGTGATATTGATACATTCTTATATAACATATCACAAGTCAAAGGATTGATTTATAAA
CAAGAAAGAGATGGTATATATCATTACCTTCTTGATCCTGGGCTCCAATTATAACGACAA
AGGTTCCAATCATGGCCAACAAAAGGTGAATCTTCATGTTCTCTCTCGATCACTATCCGG
TTTCTCTAGTACTCAAGAAAAAACAAAGACTTGCTTAGGGAAAACTGCAAAAGATATGGA
ACTGAGAGATACCTCATGTATAAGTTGGAATGAAATATACAAAAGTCAACTCCGGGTAGA
AAATTAAATAATGATCGTGGAAATAATCAATCTGGTGTTGAATAGTTGTATATGTTCAGG
AGACTCGTCATGTGTAGGAATATTTCCTCCACGAATGTATGGTTTATCGCCGAATGAT
Primers used for PCR 24wq - 8474, show primer details
   
Sequence Name 54-K118780-0022-414-D11C-24wq
GenBank Accession LN508635 [GenBank]
Sequencing primer gene specific primer, 24wq: 5'-GTTTGATTTGGTTGTTTGGTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118780-0022-414-D11C-24wq
TTTAAATGTGAACAAAAAAGAAAAAAAAATTTGTTTTCTTAGCTCATATTAGTATCTTTT
ATTTCTAGTTCGTTGTTCTTAGTTCTTTGTTCAATTGTTACAATGACTTGCCATATAGTT
TTGGCATAATGTAGTTACGTTGGAGTCTTAAAATTTTGAACACATCATTCAACAATGATG
TTTTTAACACAAGTAAAGTCAACATATCCCCTCATTCATATCTTCTCCAGAAAAAAAAAG
AGAGAAAAACCTCACATTTTTTAAAAAAATAATGTTAAATTTTATTCAAAAAAAAAACGT
CACATTGAATGCTTCAAATAGATGGACTTACCTTATAGATGCATCTGAATTGCTAAAATA
AACCCGGAAAGACATCATCAGCGAACCAAATGGTGTAACATACGTATTATTCCTTAGTGG
CCGTATTTCTATGGCTGAAATTATTGGCAAAGTTTCTCCTGTCTTAACAAGACAAATATC
CAAAGAGTTTGACCTGGCTTTGTGTATGATCTCTTCTCTGGTACCTTCTTTCTCTCCATC
CAAATCTATTCTTTTCCACTTATTGGGACCAAAATGGATATCGAAGTTTGGATCACGGTT
AAAACCATCATAATTTCCATATACAAAGCTAACCACGATGAGATAAGTTGTGCCACGCTG
GACATCAAGACTATAACAATTACGTTTTCCTTCTGGGAAGTATCTAAGAGTCAAATATTG
TTTTGATAAGTTTATGTTAAGATCTTTATCGACACTATCAATTTTTCCTGTTTGGATGAA
AGTGGAATCCGATGTCCGGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
   
Sequence Name 14-K118779-0022-414-D11C-8409
GenBank Accession LN492732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K118779-0022-414-D11C-8409
CATTTCCCGGACATCGGATTCACTTTCATCCAACAGGAAAAATTGATAGTGTCGATAAAG
ATCTTAACATAAACTTATCAAAACAATATTTGACTCTTAGATACTTCCCAGAAGGAAAAC
GTAATTGTTATAGTCTTGATGTCAAGCGTGGCACAACTTATCTCATCGTGGTTAGCTTTG
TATATGGAAATTATGATGGTCTTAACCGTGATCCAAACTTCGATATCCATCTTGGTCCCA
ATAAGTGGAAAAGAATAGATTTGGATGGAGAGAAAGAAGGTACAAGAGAAGAGATCATAC
ACAAAGCCAGGTCAAACTCTTTGGATATTTGTCTTGTTAAGACAGGAGAAACTTTGCCAA
TAATTTCAGCCATAGAAATACGGCCACTAAGGAATAATACGTATGTTACACAATCTGGTT
CGCTGATGATGTCTTTCCGGGTTTATCTTAGCAATTCAGATGCATCTATAAGGTAAGTCA
ATCTATTTGAAGCATTCAATGTGACGTTTTTCTTTTGAATAAAATTTAACATTATTCTTT
TAAAAAATGTGAGGTTTTTCTCTCTTTTTTTTTCTGGAGAAGATATGAATGAGGGGATAT
GTTGACTTTACTTGTGTTAAAAACATCATTGTTGAATGATGTGTTCAAAATTTTAAGACT
CCAACGTAACTACATTATGCCAAAACTATATGGCAAGTCATTGTAACAATTGAACAAAGA
ACTAAGAACAACGAACTAGAAATAAAAGATACTAATATGAGCTAAGAAAACAAATTTTTT
TTTTCTTTTTTTTGTTCACAATTAAAACTAATTTAATTAATTGAA

Last Updated on 06.12.2018 16:11