Sequences from confirmation amplicon


Confirmation sequences for line 414E07 and hit At5g57440 are shown.
Line ID 414E07
Locus At5g57440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23272523
Primers used for PCR CC13 - 8474, show primer details
   
Sequence Name 36-K105566-0022-414-E07C-CC13
GenBank Accession LN502224 [GenBank]
Sequencing primer gene specific primer, CC13: 5'-GGAGACTGTTGCCTGTTAGTTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K105566-0022-414-E07C-CC13
CCGTCAGAGCAGGCTTTCCTTGCTTCCCTCTGGATCATCACCACGAACTACGTGATGCAT
CAATGAGAAAAGCTCTCTGTGTCTTTGGGTTTTCAGATCATAATGCCGTGTGTGAGTACT
GCAAGCGTACAATCAAACATAACAAGTCAGTTTCTGGATTGTAAAGGACAATGAGAATGC
AAACTAAATGGTATATTATGAGATGAGGTGAAGAGATTACCCGGTAGCAATACATATTGG
AATATTCTTTACATGGAGATGCTTAATGAGTCTACTAGCCCCTGAAAATGTGGAAGAGAA
TTGGTTCATGACCACATCAAAAACATAAATATCCGTACATGTAATCTTCCTGTGAACCAA
ATGTCATGTGTTACAAAAAGCAGAATCTACCTGGCATGAGCTCACTAGTAGGGAAAAGGT
CTTGCAACATTGACTCTCTCTCGACAAGAAAGTCCTCAGCTGAAAGAGAGTCGCTAATCC
CGCTTTCTTCAACAAAGATCCTGGCAGCTTCTATTGCTTTTCTACCCATCATTTTAGCCT
TCAATTGAAAAAGAAAAATGGCTTCACGTCCGGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 68-K105567w-0022-414-E07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K105567w-0022-414-E07C-8409
GAGCCTTTACAATTGAATATATCCCGGACGTGAAGCCATTTTTCTTTTTCAATTGAAGGC
TAAAATGATGGGTAGAAAAGCAATAGAAGCTGCCAGGATCTTTGTTGAAGAAAGCGGGAT
TAGCGACTCTCTTTCAGCTGAGGACTTTCTTGTCGAGAGAGAGTCAATGTTGCAAGACCT
TTTCCCTACTAGTGAGCTCATGCCAGGTAGATTCTGCTTTTTGTAACACATGACATTTGG
TTCACAGGAAGATTACATGTACGGATATTTATGTTTTTGATGTGGTCATGAACCAATTCT
CTTCCACATTTTCAGGGGCTAGTAGACTCATTAAGCATCTCCATGTAAAGAATATTCCAA
TATGTATTGCTACCGGGTAATCTCTTCACCTCATCTCATAATATACCATTTAGTTTGCAT
TCTCATTGTCCTTTACAATCCAGAAACTGACTTGTTATGTTTGATTGTACGCTTGCAGTA
CTCACACACGGCATTATGATCTGAAAACCCAAAGACACAGAGAGCTTTTCTCATTGATGC
ATCACGTAGTTCGTGGTGATGATCCAGAGGTGAAGCAAGGAAAGCCTGCTCCTGACGGGT
TTCTCGCTGCTGCTAGGAGATTTAAGGTAACTAA
Primers used for PCR CC14 - 8474, show primer details
   
Sequence Name 37-K105566-0022-414-E07C-CC14
GenBank Accession LN502615 [GenBank]
Sequencing primer gene specific primer, CC14: 5'-AACTAACTTCCGACTGAAACTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K105566-0022-414-E07C-CC14
ATCACAATGTCGAATCCTGCAGCCGTCACCGCCGGCAGAGGATCAATCACTCACGTCATA
TTCGACATGGACGGTCTTCTTCTCGGTAATCTCCTTGCATCTGATCTTCAATCTCCAGCT
TTATTAGATCGTACTACTGTCACTATCGTTGTTTAGATTTGATGGTTTTTTTAGTAGTGA
CTGTTTAGTTTCCAGGATTGATTTCATACAATTGATGTTAAGATGCTAAAGCAATTAGCT
TGAATCTGATCGGAAATCGAGCGATTGAGTTTTTGTTCTGTGTATTCAAAATCTCACAAC
TTCATTGGCTTATCTGCTCTTGCTGCATACATTCATGCTGATTTGGTTTCTGTTGCAGAT
ACTGAGAAGTTTTACACTGAGGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 69-K105567w-0022-414-E07C-OriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K105567w-0022-414-E07C-OriL
GCCATTTACATTGACCTCAGTGTAAAACTTCTCAGTATCTGCAACAGAAACCAAATCAGC
ATGAATGTATGCAGCAAGAGCAGATAAGCCAATGAAGTTGTGAGATTTTGAATACACAGA
ACAAAAACTCAATCGCTCGATTTCCGATCAGATTCAAGCTAATTGCTTTAGCATCTTAAC
ATCAATTGTATGAAATCAATCCTGGAAACTAAACAGTCACTACTAAAAAAACCATCAAAT
CTAAACAACGATAGTGACAGTAGTACGATCTAATAAAGCTGGAGATTGAAGATCAGATGC
AAGGAGATTACCGAGAAGAAGACCGTCCATGTCGAATATGACGTGAGTGATTGATCCTCT
GCCGGCGGTGACGGCTGCAGGATTCGACATTGTTGATTCGATACCGATAACGACGAACCG
AATCCAGTTTCAG

Last Updated on 06.12.2018 16:11