Sequences from confirmation amplicon


Confirmation sequences for line 414F03 and hit At1g64100 are shown.
Line ID 414F03
Locus At1g64100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23792230
Primers used for PCR 23op - 8474, show primer details
   
Sequence Name 30-K114909-0022-414-F03C-23op
GenBank Accession LN499841 [GenBank]
Sequencing primer gene specific primer, 23op: 5'-AGTAGGAGACTGATTCATGGTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114909-0022-414-F03C-23op
ACTTTTGAAGGAGAGGAACTGAAATTGAAAAGTGGTTCTCATTATTTCAAAAGCTTAGAT
GATGCCATTGATTTCTTCGATTACATGGTTAGATCTCGTCCCTTCTATACGGCAGTTGAT
TGCAATAAAGTAATTGGTGTCTTCGTGAGAATGAATCGTCCCGATGTTGCGATTTCTCTC
TACCGGAAGATGGAAATTTCGCCGGATTCCACTTAATATCTACAGCTTCAATATTCTGAT
AAAATGTTTCTGCGACTGCCATAAGTTGTCTTTTTCTTTGTCTACATTTGGCAAGCTCAC
TAAACTTGGTTTCCAGCCCGATGTTGTTACCTTTAACACGCTGCTCCACGGCTTATGTCT
CGAAGATAGGATTTCTGAAGCTTTAGCTTTGTTTGGTTATATGGTTGAAACTGGATGTCA
ACCGGATGTCGTTACCTTCAACACACTTATGAACGGTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 06-K114908-0022-414-F03C-8409
GenBank Accession LN488927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114908-0022-414-F03C-8409
TGTTGAAGGTAACGACATCCGGTTGACATCCAGTTTCAACCATATAACCAAACAAAGCTA
AAGCTTCAGAAATCCTATCTTCGAGACATAAGCCGTGGAGCAGCGTGTTAAAGGTAACAA
CATCGGGCTGGAAACCAAGTTTAGTGAGCTTGCCAAATGTAGACAAAGAAAAAGACAACT
TATGGCAGTCGCAGAAACATTTTATCAGAATATTGAAGCTGTAGATATTAAGTGGAATCC
GGCGAATTTCCATCTTCCGGTAGAGAGAAATCGCAACATCGGGACGATTCATTCTCACGA
AGACACCAATTACTTTATTGCAATCAACTGCCGTATAGAAGGGACGAGATCTAACCATGT
AATCGAAGAAATCAATGGCATCATCTAAGCTTTTGAAATAATGAGAACCACTTTTCAATT
TCAGTTCCTCTCCTTCAAAAGTAACTTGGGTCAAAGAAGGCAGACTCTTTGTACCTTTCT
CGGCGACACGACCATGA

Last Updated on 06.12.2018 16:11