Sequences from confirmation amplicon


Confirmation sequences for line 415G02 and hit At5g61730 are shown.
Line ID 415G02
Locus At5g61730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24804218
Primers used for PCR 21ci - 8474, show primer details
   
Sequence Name 60-K107936-0022-415-G02C-21ci
GenBank Accession LN510279 [GenBank]
Sequencing primer gene specific primer, 21ci: 5'-ACAGGTATCTGCACATTGAGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K107936-0022-415-G02C-21ci
TTATTAGTTCTAATTCTCATAGTGCATTTCATCGACGGTTACATGTGAAACTGATTATTT
TGATGGACCTTGTAGACTAATTACCTCGACTGCGATGCCTGATTCTAACTCGAGAGTTAC
CATAGTTCCTTCAACAGTTGCGCTTTCTAGTTCAGCACGTCTAGCGATGTTCAAAAACAC
TTCTTCAAGAGTGGCAAGACCGAGCTGAATGTCTGCGATACCAAATTCAGATTCTCTATC
TTGTAGCTCCGCGAAAAACCCCTATTCCCGTTAATTATGGAAAAATCATTCATCAAATGA
AAGATCTAAGAGATAATTAATAAAGCTTTTTATAGATGGTTACCTTCAAAAGTTGCTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
ACGGTTATTA
   
Sequence Name 44-K107935-0022-415-G02C-8409
GenBank Accession LN505206 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K107935-0022-415-G02C-8409
GCATTTACATTGAGCAACTTTTGAAGGTAACCATCTATAAAAAGCTTTATTAATTATCTC
TTAGATCTTTCATTTGATGAATGATTTTTCCATAATTAACGGGAATAGGGGTTTTTCGCG
GAGCTACAAGATAGAGAATCTGAATTTGGTATCGCAGACATTCAGCTCGGTCTTGCCACT
CTTGAAGAAGTGTTTTTGAACATCGCTAGACGTGCTGAACTAGAAAGCGCAACTGTTGAA
GGAACTATGGTAACTCTCGAGTTAGAATCAGGCATCGCAGTCGAGGTAATTAGTCTACAA
GGTCCATCAAAATAATCAGTTTCACATGTAACCGTCGATGAAATGCACTATTGAGAATTA
ATGAACTTGAATTTAAATGGATACAAACTAACCTCAATGTGCAGATACCTG

Last Updated on 06.12.2018 16:11