Sequences from confirmation amplicon


Confirmation sequences for line 416C06 and hit At5g57410 are shown.
Line ID 416C06
Locus At5g57410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23265255
Primers used for PCR 32tv - 8474, show primer details
   
Sequence Name 29-K117118-0022-416-C06C-32tv
GenBank Accession LN499455 [GenBank]
Sequencing primer gene specific primer, 32tv: 5'-AAGGAGAGTTTTGGTGGTAGTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117118-0022-416-C06C-32tv
CTTTAGCACTTGGATCCCTGATTTTGAGCTCCTAGACATTTTCATACTCCCTGTAGAGAT
ACATTATATAAATACAGATCAAGCAAGAGACATTACTAATCTTTTTACAAAGAAAAAACG
TACAGGAGTAATTAAGGAAGAGGAATCTATAAGTATACCTCAGCTGCCAACGAAGCTACA
GATTCCCTGCAACAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K117117-0022-416-C06C-8409
GenBank Accession LN489447 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117117-0022-416-C06C-8409
ATTTCATTGAATATATCATTGTTGCAGGGAATCTGTAGCTTCGTTGGCAGCTGAGGTATA
CTTATAGATTCCTCTTCCTTAATTACTCCTGTACGTTTTTTCTTTGTAAAAAGATTAGTA
ATGTCTCTTGCTTGATCTGTATTTATATAATGTATCTCTACAGGGAGTATGAAAATGTCT
AGGAGCTCAAAATCAGTGGATCCAAGTTGCTAAAAGAGACTTGTCGCAAAATGTAGTCGT
AGTACTGTATTCACTACCACCAAAACTCTCCTT

Last Updated on 06.12.2018 16:11