Sequences from confirmation amplicon


Confirmation sequences for line 417B05 and hit At1g62520 are shown.
Line ID 417B05
Locus At1g62520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23144694
Primers used for PCR AB69 - 8474, show primer details
   
Sequence Name 38-K105566-0022-417-B05C-AB69
GenBank Accession LN502979 [GenBank]
Sequencing primer gene specific primer, AB69: 5'-ATTATGAGAAAACAAGGCAACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105566-0022-417-B05C-AB69
CTCACTGAGTCAACCGTGACCTCAAATCCGAGTCATACTTTAACTCGTTTTGCTTAGTGA
CCCGACCCGCTATGACCCGACAAACCAACATTGCCTTCCTCCCTTTCCCACCGCCAGCTT
TCTCATTCGCCGTGCTACTTCCGGCAAACGTATATATCGACGCGCCACCGCCTTTTCCTC
CCAAAATACCCCACGCGCTGCCACCTCCACCGTAACTTGGACCCAAACAGTAAAACCTCA
TTGTTTCATTCCCGTCGGCAACCGAACGCGCGTTCTCTTCACGCGCTTTCCCCACGCTCC
TAGCTTTCACGGCTTCACGATACTCTTCGAACCGGGTCAAAGTCTTAGACCCGTTTTGCA
CTTTGAAAATCATTTCGACCCGACCCGAAAACGGTTTCGGACCCCAGCTCGTCTGAAAGA
TAATCTCCACCACATTACGTGACTGATGACCTTCCGATAGCTCCGTCAATGTCGGTAAAA
ACGGATCACGATCACGAACTCCGGTTGATCGGATCCGGGTTGATGGTGATAAAACCGGGT
CGGTTTCTCGTTTATCCGGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 70-K105567w-0022-417-B05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K105567w-0022-417-B05C-8409
GCCTTTCATTGAATATATCCTGCCCGGATAACGAGAAACCGACCCGGTTTTATCACCATC
AACCCGGATCCGATCAACCGGAGTTCGTGATCGTGATCCGTTTTTACCGACATTGACGGA
GCTATCGGAAGGTCATCAGTCACGTAATGTGGTGGAGATTATCTTTCAGACGAGCTGGGG
TCCGAAACCGTTTTCGGGTCGGGTCGAAATGATTTTCAAAGTGCAAAACGGGTCTAAGAC
TTTGACCCGGTTCGAAGAGTATCGTGAAGCCGTGAAAGCTAGGAGCGTGGGGAAAGCGCG
TGAAGAGAACGCGCGTTCGGTTGCCGACGGGAATGAAACAATGAGGTTTTACTGTTTGGG
TCCAAGTTACGGTGGAGGTGGCAGCGCGTGGGGTATTTTGGGAGGAAAAGGCGGTGGCGC
GTCGATATATACGTTTGCCGGAAGTAGCACGGCGAATGAGAAAGCTGGCGGTGGGAAAGG
GAGGAAGGCAATGTTGGTTTGTCGGGTCATAGCGGGTCGGGTCACTAAGCAAAACGAGTT
AAAGTATGACTCGGATTTGAGGTCACGGTTTGACTCAGTGAGTGGTGACGACGGAGAGTT
GCTCGTGTTCGATACACGTGCGGTGTTGCCTTGTT
Primers used for PCR AR05 - 8474, show primer details
   
Sequence Name 30-K118837-0022-417-B05C-AR05
GenBank Accession LN499930 [GenBank]
Sequencing primer gene specific primer, AR05: 5'-TAAAAACAATAAAATCCGGCTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118837-0022-417-B05C-AR05
ATTTCGTCTTGTTTTTCTGCATTACCTTACATAAAATCAAAAAACAAATTTTCCATTCTT
ATAATTTTTTTGAAAAAACTTAAAATCAATAAGACAAAAGTGATATGAAGGAATGGGGTC
ATATCGTAGAATCATCTTACATAAATCAAAAAAATAAATTTATGTTGTAAAAAATTACTA
TGTTATATTAAACTAAAGGGTAATACCGTAATTTACACTCCCAAGTTTCGACGTACACTT
TTAATATTCATCATCATCATCGCGCCATGTTGCTTTCTTCTTCTGCTTCTGTTTCTTTTC
CAAGTTTTTAAAGAGAGAGAGAGAGACTGAAAGAAAAACAAAAAACAGAGAGCTTTGGTT
CCGGATCTTGATTATGGCAAATGGGTGGGTCAAATCGTTACAATGCAAGTCGAAAGCTTT
CGATGATGTTTACAATCCGATTAAAAACCCGAACCCGAATCCGAGCCCGAGTCCGAAACA
TCTCATGTCAAGCTACAGTTGCAGAAAAAGCACACAGAGTCTAAAAGATGTGATCGAAAC
CAAAAAACCCAAACCTAAACCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K118836-0022-417-B05C-8409
GenBank Accession LN489023 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118836-0022-417-B05C-8409
TCGGATGAGCCATTTCATTGAATATATCCTGTCCGGTTTAGGTTTGGGTTTTTTGGTTTC
GATCACATCTTTTAGACTCTGTGTGCTTTTTCTGCAACTGTAGCTTGACATGAGATGTTT
CGGACTCGGGCTCGGATTCGGGTTCGGGTTTTTAATCGGATTGTAAACATCATCGAAAGC
TTTCGACTTGCATTGTAACGATTTGACCCACCCATTTGCCATAATCAAGATCCGGAACCA
AAGCTCTCTGTTTTTTGTTTTTCTTTCAGTCTCTCTCTCTCTCTTTAAAAACTTGGAAAA
GAAACAGAAGCAGAAGAAGAAAGCAACATGGCGCGATGATGATGATGAATATTAAAAGTG
TACGTCGAAACTTGGGAGTGTAAATTACGGTATTACCCTTTAGTTTAATATAACATAGTA
ATTTTTTACAACATAAATTTATTTTTTTGATTTATGTAAGATGATTCTACGATATGACCC
CATTCCTTCATATCACTTTTGTCTTATTGATTTTAAGTTTTTTCAAAAAAATTATAAGAA
TGGAAAATTTGTTTTTTGATTTTATGTAAGGTAATGCAGAAAAACAAGACGGAAATAAAT
GTAAGATAAAAATTGACAGCCGGATTTTATTTGTTTTTA

Last Updated on 06.12.2018 16:11