Sequences from confirmation amplicon


Confirmation sequences for line 417E05 and hit At2g43445 are shown.
Line ID 417E05
Locus At2g43445 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18042256
Primers used for PCR 34yb - 8474, show primer details
   
Sequence Name 19-K119666-0022-417-E05C-34yb
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 34yb: 5'-GTTCACTAATTGGGTATCTTGCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119666-0022-417-E05C-34yb
CGTTTCTTGGTGAGATCCAGGCCAGTATTATGTAACTCACACTAATTTGTAACCAGTAGA
TTGATCCATTCACACACACCGATTTACTTCCCACTCGCATTATATGAGGAGGTGACATCA
GTTTGCTCCATTCGCCAGATTCAATATCAAGAATCCCAAATTCATAACAATCATGAAGCC
ATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGC
   
Sequence Name 03-K119665-0022-417-E05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119665-0022-417-E05C-8409
ATTTCATTGAATATGATGGCTTCATGATTGTTATGAATTTGGGATTCTTGATATTGAATC
TGGCGAATGGAGCAAACTGATGTCACCTCCTCATATAATGCGAGTGGGAAGTAAATCGGT
GTGTGTGAATGGATCAATCTACTGGTTACAAATTAGTGTGAGTTACATAATACTGGCCTT
GGATCTTCACCAAGAAACGTTCAATGGTGTCTACCACCTCCCGGCTACGTGGGTCACGCA
AGATACCCAATTAGTGAA

Last Updated on 06.12.2018 16:11