Sequences from confirmation amplicon


Confirmation sequences for line 417H05 and hit At1g13250 are shown.
Line ID 417H05
Locus At1g13250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4530026
Primers used for PCR JG68 - 8474, show primer details
   
Sequence Name 75-K034070-022-417-H05C-JG68
GenBank Accession LN513183 [GenBank]
Sequencing primer gene specific primer, JG68: 5'-TCCGATGTTACAATCAGCTATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K034070-022-417-H05C-JG68
ATAAGGAGCCCATAACGAATCCAACGGACACGGTCGTCTCGAGTCAAGCCTTAGCCATGG
TTTCCCTTTCCCGCTCCAATGCAACAAACTCACCGGACCTGGATGCAAATTCCGGCAAAG
TCCTTCCAAGTTATCACCACCAAGACCATGCTGATTCCACCTATGCTCCACCGGCTCAAC
ATCTCCGGCGAAAACGAGCAGAAACGGAGGCAAAGATCCCAATTCGTAAATACGATGTCG
TTTTTGAATCCTCATCCATGTCTCTAGCTTCACCGTGACTCTCCTTTCTCTCCATTTTCC
GAGATCAATCACCATCACTCCGGTGTTGAAATAACACGGTTTCCTATCTTTCAACGCCGA
TTTGTAACCTTGACTCGACCAGAATCTTGAAGTGAAGTAGTTAGTGAAATTCGCGTGACA
GTACTCCGGAGCTCCGACGACGTGCCGACGTAGATCGATTCTCCAGAGTTTAGCCACGTC
ATCGACGACTACGAGATCGGAGTCGAAGTAGATTACGCGGCGGACGGCGATTGGGAGGAG
ATCGGCGAGGTAGATCCGAGCGTAGTTTAACGGTTGGTCTAAAGCACGACGAATAGAGGA
AGATATTTTGCTGCGGACGAGGTTAGGGTCAAAATGGTAAATGTGGTAGGTTAGGTATGG
GAATGTTGAGGAGATTATGCGGCGGAGATCGGCGCTGCGACGGTGAGTGGCGATGAAGTG
GAAAACGATGTTTTCAGGACAAGAAGCGTGTTGGAGAACGGAGAAGACGCCGGCGACTGA
GCCACGGAGGTAAATTGCGTCGAGAGTCATAGCGATGTGGATGATTGATGGGTTGTGTTC
GTGATCCGAAGGTATCCATGTCGTTTTGGAGCATTCTCTGCCGTTTCGAAACGCCGGAGC
TTCACGAAACGCGGGGAGGTCAGTGAGAGTGACTGTGACGCAGCTAATTGTGATAG
   
Sequence Name 59-K034070-022-417-H05C-8409
GenBank Accession LN509918 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K034070-022-417-H05C-8409
GAAGCCATTTACTACACAACACAGACCTAAAAAAATGTCTTCTCTGCGTTTGCGTTTATG
TCTTCTTCTACTCTTACCTATCACAATTAGCTGCGTCACAGTCACTCTCACTGACCTCCC
CGCGTTTCGTGAAGCTCCGGCGTTTCGAAACGGCAGAGAATGCTCCAAAACGACATGGAT
ACCTTCGGATCACGAACACAACCCATCAATCATCCACATCGCTATGACTCTCGACGCAAT
TTACCTCCGTGGCTCAATCGCCGGCGTCTTCTCCGTTCTCCAACACGCTTCTTGTCCTGA
AAACATCGTTTTCCACTTCATCGCCACTCACCGTCGCAGCGCCGATCTCCGCCGCATAAT
CTCCTCAACATTCCCATACCTAACCTACCACATTTACCATTTTGACCCTAACCTCGTCCG
CAGCAAAATATCTTCCTCTATTCGTCGTGCTTTAGACCAACCGTTAAACTACGCTCGGAT
CTACCTCGCCGATCTCCTCCCAATCGCCGTCCGCCGCGTAATCTACTTCAACTCCGATCT
CGTAGTCGTCGATGACGTGGCTAAACTCTGGAGAATCGATCTACGTCGGCACGTCGTCAG
AGCTCCGGAGTACTGTCACGCGAATTTCACTAACTACTTCACTTCAAGAATTCTGGTAGA
GTCAAGGTTACAAATCGGCGTTGAAAGATAGGAAACCGTGTTATTTCAACACCGGAGTGA
TGGTGATTGATCTCGGAAAATGGAGAGAAAGGAGAGTCACGGTGAAGCTAGAGACATGGA
TGAGGATTCAAAAACGACATCGTATTTACGAATTGGGATCTTTGCCTCCGTTTCTGCTCG
TTTTCGCCGGAGATGTTGAGCCGGTGGAGCATAGGTGGAATCAGCATGGTCTTGGTGGTG
ATAACTTGGAAGGACTTTGCGGGAATTTGCATCCAGGTCCGGTGAGTTTTGTGCATTGGA
GCGGGAAAGGGAAACCATGGCTAAGGCTTGACTCGAGACG

Last Updated on 06.12.2018 16:11