Sequences from confirmation amplicon


Confirmation sequences for line 417H11 and hit At5g47310 are shown.
Line ID 417H11
Locus At5g47310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19202053
Primers used for PCR NU05 - 8474, show primer details
   
Sequence Name 29-K118313-0022-417-H11C-NU05
GenBank Accession LN499477 [GenBank]
Sequencing primer gene specific primer, NU05: 5'-AATTATCTCTATTGGTTCGGCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118313-0022-417-H11C-NU05
TGGCTTCTTCTCTATACTTACTTGACCTAACTAAATTTTTCTTTGTCATTGCTTTCTATT
TGCTTTCATCCATATCTGGTTTATCATGTTATCAAAGTACTTCTAGCCTTGAATAGGAAC
AATGATATTCCTCAATTGTTCTCCACAAGTTGTGATTTATTATTCATTAGGTGAAAATGG
TGTTTATTATATTAGCTATGGAATACATTATTAGAAGGAAAAAGTGGAACAAGTTTTGAG
GAATCTAGAATGGAATGCTTTTTCAAATCCTGCCTTATTGTCTGAAGCATTCTCTTTCAA
TAATTAGAACTTGCCTGATGAGTTATATAACTGTACTTCTGCTGAAACTGTTATTGTTGC
TAAATGTTCATCTAATGTGGAGTTTTTTTTCACTTTGCCATGTGTACTACTAGCTCATGG
TTTCGAATATGGATATGGAGCTCATGAGTATTCGAGCAGTGGGGTATTTGAGGTTGAACC
TAGGAGCTGTCCAGGTTTCATCTTTAGGCGCTCAGTTTTGTTGGGAACCACCAGCATGTC
TCGCTCTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTC
   
Sequence Name 15-K118311-0022-417-H11C-8409
GenBank Accession LN493178 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118311-0022-417-H11C-8409
TCAGAAGCGAGACATGCTGGTGGTTCCCAACAAAACTGAGCGCCTAAAGATGAAACCTGG
ACAGCTCCTAGGTTCAACCTCAAATACCCCACTGCTCGAATACTCATGAGCTCCATATCC
ATATTCGAAACCATGAGCTAGTAGTACACATGGCAAAGTGAAAAAAAACTCCACATTAGA
TGAACATTTAGCAACAATAACAGTTTCAGCAGAAGTACAGTTATATAACTCATCAGGCAA
GTTCTAATTATTGAAAGAGAATGCTTCAGACAATAAGGCAGGATTTGAAAAAGCATTCCA
TTCTAGATTCCTCAAAACTTGTTCCACTTTTTCCTTCTAATAATGTATTCCATAGCTAAT
ATAATAAACACCATTTTCACCTAATGAATAATAAATCACAACTTGTGGAGAACAATTGAG
GAATATCATTGTTCCTATTCAAGGCTAGAAGTACTTTGATAACATGATAAACCAGATATG
GATGAAAGCAAATAGAAAGCAATGACAAAGAAAAATTTAGTTAGGTCAAGTAAGTAATAG
AGAAGAAGCCACAATTACCCTCAATGCCAGAGTGAAATATGCCAAGGCCG

Last Updated on 06.12.2018 16:11