Sequences from confirmation amplicon


Confirmation sequences for line 419A11 and hit At2g38150 are shown.
Line ID 419A11
Locus At2g38150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15982901
Primers used for PCR 08wb - 8474, show primer details
   
Sequence Name 25-K118356-0022-419-A11C-08wb
GenBank Accession LN497659 [GenBank]
Sequencing primer gene specific primer, 08wb: 5'-TTAAGGATAGTGTATCCGTTTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118356-0022-419-A11C-08wb
GCCAGCAGCACCAGGATTGGTGGTGAAGAGAGTATCAATAGCTAACATCTCTCTTGGTCC
AAAAGAATTTGCAGGAGAAAGCCAAATCATAAAGAACTGAGCAGAGCAATTCTTGTTGTA
CAAGTCCAAAACTCTTGTATGGAAACTCTTGCTCTTGGTAGTCGATTTCAGTATCTCCAG
CTCAGGTAGCTTTCTTCTGAACCAGTCAATTCTCTGGTTCTTGCTAGCTTTACGAGGAGG
TAACAACGGATCTAACTCCTTCTCTTTATGCTCCGAGTCCACAACTGAGTTGATATTACA
ACTACTACTCATGGGGCCATAGGGTCTGGGAAGAGGCACGTTTGGTTTGAACGGTAGGTT
ATTGTCATAGAGAGTTGATTTTGGAGATTCTATATAGAGGAGATACAATAATAATACAGA
AAACGTGAAGTTCGCGAAGATCGATAGATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTATT
   
Sequence Name 25-K118355-0022-419-A11C-8409
GenBank Accession LN497658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118355-0022-419-A11C-8409
TACATTGAATCTATCGATCTTCGCGAACTTCACGTTTTCTGTATTATTATTGTATCTCCT
CTATATAGAATCTCCAAAATCAACTCTCTATGACAATAACCTACCGTTCAAACCAAACGT
GCCTCTTCCCAGACCCTATGGCCCCATGAGTAGTAGTTGTAATATCAACTCAGTTGTGGA
CTCGGAGCATAAAGAGAAGGAGTTAGATCCGTTGTTACCTCCTCGTAAAGCTAGCAAGAA
CCAGAGAATTGACTGGTTCAGAAGAAAGCTACCTGAGCTGGAGATACTGAAATCGACTAC
CAAGAGCAAGAGTTTCCATACAAGAGTTTTGGACTTGTACAACAAGAATTGCTCTGCTCA
GTTCTTTATGATTTGGCTTTCTCCTGCAAATTCTTTTGGACCAAGAGAGATGTTAGCTAT
TGATACTCTCTTCACCACCAATCCTGGTGCTTGCTTGGCGATTTTGTCCAACTCCTTAGA
CTCCCCAAACGGATA

Last Updated on 06.12.2018 16:11