Sequences from confirmation amplicon


Confirmation sequences for line 419E07 and hit At3g15570 are shown.
Line ID 419E07
Locus At3g15570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5271679
Primers used for PCR 50ns - 8474, show primer details
   
Sequence Name 78-K114626-0022-419-E07C-50ns
GenBank Accession LN513725 [GenBank]
Sequencing primer gene specific primer, 50ns: 5'-ATACTCTGGTTTCAAGCTCCGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K114626-0022-419-E07C-50ns
AAATTGCAAGGAACAGAGTCTTTCTCCGGTGGGAGGATTCCGATTAGTGTTTCGACGAAA
AACCGTTTCTTCATTACAGATGCAGTGACGCTCTCTGGCTGTGTGTCATTGTGGTTGTTC
TTGGATTCTAAGGAGGTGGAGGAAATAATGGCGGAGACATTGCCGGAGAGATCAGGGAGC
CATTTTGAAGCGTAATGAGCGATGATGGAACCGATAAGGTCTGGTCTGATTCCTTTGGAT
TTGATGCCGGTGATTGTTTTGACGAAATAGTTCATGTCTTGGATACAAGATTCGTCGAAG
TAAGTGTAGTACTCCATATCACCGACGAAGGTTTCTTGGCGATTGCCGTAGATGGTGACG
GGTTCATTGCTGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTATATGTCCGCACGGATTATA
   
Sequence Name 78-K114625-0022-419-E07C-8409
GenBank Accession LN513724 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K114625-0022-419-E07C-8409
TTGAATATATCCTGGATCAGCAATGAACCCGTCACCATCTACGGCAATCGCCAAGAAACC
TTCGTCGGTGATATGGAGTACTACACTTACTTCGACGAATCTTGTATCCAAGACATGAAC
TATTTCGTCAAAACAATCACCGGCATCAAATCCAAAGGAATCAGACCAGACCTTATCGGT
TCCATCATCGCTCATTACGCTTCAAAATGGCTCCCTGATCTCTCCGGCAATGTCTCCGCC
ATTATTTCCTCCACCTCCTTAGAATCCAAGAACAACCACAATGACACACAGCCAGAGAGC
GTCACTGCATCTGTAATGAAGAAACGGTTTTTCGTCGAAACACTAATCGGAATCCTCCCA
CCGGAGAAAGACTCTGTTCCTTGCAATTTCCTCCTCCGTCTCCTCAGGACAGCGAAAATG
GTCGGAGCTAATCCGAATTATTTAACGGAGCTAGAACCCAGAGTA

Last Updated on 06.12.2018 16:11