Sequences from confirmation amplicon


Confirmation sequences for line 419E07 and hit At4g22900 are shown.
Line ID 419E07
Locus At4g22900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12010522
Primers used for PCR 32qv - 8474, show primer details
   
Sequence Name 43-K115781-0022-419-E07C-32qv
GenBank Accession LN504953 [GenBank]
Sequencing primer gene specific primer, 32qv: 5'-AAAACCTCTACGTTAGAACGGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K115781-0022-419-E07C-32qv
AGGTAAAAGAGAATCTGACAAATGAAGTGTAAGTGTTTATAATGTTTGTGTCCCAAAACA
CACATCCCCCACAAAAGTAAAAACACCATCAAAACTTTCTTTCTTTCCTCTTATCTTCAC
TTCCTCTTGTAAACCCTAGATTCTCTTGTTCCTCTTATGGATTCATTCTCTTTTTGTATC
ATCATCATGATACTTCTCTTATCATTCCACCAATCTAAGTCTCAATTGATTCAATCTACA
CATCTTCTTGATCTCATGATCAGAGATTACACCATTAGAAACTTTAAGCTCAATTTCAAC
ACAGGCGTCACTCAGAAAATCTATCTCCCTTCTAATTTCTCTGGTATCGATATTGACACG
GTCAAGTTAAGGTGTGGGAGTTTGAGAAGATATGGCGCAAAAATTGGAGAGTTTCACATC
GGTTCAGGCCTTACGGTGGAGCCATGTCCGGAGAGAGTAATTACCAATGGCTTCATGTAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATTCC
   
Sequence Name 19-K115780-0022-419-E07C-8409
GenBank Accession LN494901 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115780-0022-419-E07C-8409
ACATGAAGCCATTGGTAATTACTCTCTCCGGACATGGCTCCACCGTAAGGCCTGAACCGA
TGTGAAACTCTCCAATTTTTGCGCCATATCTTCTCAAACTCCCACACCTTAACTTGACCG
TGTCAATATCGATACCAGAGAAATTAGAAGGGAGATAGATTTTCTGAGTGACGCCTGTGT
TGAAATTGAGCTTAAAGTTTCTAATGGTGTAATCTCTGATCATGAGATCAAGAAGATGTG
TAGATTGAATCAATTGAGACTTAGATTGGTGGAATGATAAGAGAAGTATCATGATGATGA
TACAAAAAGAGAATGAATCCATAAGAGGAACAAGAGAATCTAGGGTTTACAAGAGGAAGT
GAAGATAAGAGGAAAGAAAGAAAGTTTTGATGGTGTTTTTACTTTTGTGGGGGATGTGTG
TTTTGGGACACAAACATTATAAACACTTACACTTCATTTGTCAGATTTCTCTTTTACCTT
TATAGAAGACATGATCTAATTGTTAAATAAGCATATGACCGTTC

Last Updated on 06.12.2018 16:11